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The protective effect of quercetin in the alcohol-induced liver and lymphoid tissue injuries in newborns

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Abstract

Recently published experimental and clinical studies indicate that oxidative stress leads to the pathogenesis and progression of alcohol-induced tissue injuries. Quercetin is a type of flavonoid compound that influences antioxidant and anti-inflammatory activities have protective and therapeutic effects for treating various diseases including diabetes mellitus and neuro-degenerative diseases. In this study, fetal alcohol syndrome was tested in rat models, with the aim of verifying the protective effect of quercetin in preventing alcohol-induced liver and lymphoid tissue (thymus, spleen, and lymph nodes) injuries on the 21st day for the offspring of alcohol treated mother rats. The pregnant rats were randomly assigned into four groups. The control group (C) (n = 3) of pregnant rats received only physiological saline intraperitoneally (i.p.) throughout the pregnancy (1 to 21 days gestation) and during lactation until postnatal day 21. The quercetin positive control group (QT) of pregnant rats (n = 3) received quercetin at 50 mg/kg/days i.p. for the same period. The ethanol treatment group (E) (n = 3) of pregnant rats received 1 ml/day of 40% v/v ethanol (4 g/kg) intragastrically (i.g) for the same period. The model group of pregnant rats (EQ) received ethanol + quercetin (n = 3) with a dose of 1 ml/day of v/v ethanol (4 g/kg i.g.) and quercetin at 50 mg/kg body weight per day i.p. for the same period. Ten offspring were used in each of the C, QT, E and EQ groups. Malondialdehyde (MDA), protein carbonyl content (PC) and chemiluminescence levels (CL) in liver and lymphoid tissues significantly increased in group E versus the C group (P < 0.05–P < 0.001) whereas glutathione levels (GSH), glutathione reductase (GR), glutathione peroxidase (GP), superoxide dismutase (SOD), and catalase (CAT) activities significantly decreased in group E compared to the C group (P < 0.05–< 0.001). However, tissue MDA, PC, and CL levels decreased in the EQ group compared to group E. GSH level, GP, GR, SOD, and CAT activity were significantly increased by quercetin (P < 0.05–P < 0.001). The plasma TNFα, IL-1β, and IL-6 levels and NF-κB activation significantly increased in group E compared to the C and QT groups, but IL-10 significantly decreased in group E compared to the C and QT groups. The TNFα, IL-1β, and IL-6 levels and NF-κB activation significantly decreased in group EQ compared to group E. In conclusion, quercetin has a protective effect against maternal alcohol-induced oxidative and inflammatory damage in the liver and lymphoid tissues of newborn rats.

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Abbreviations

FASD:

Fetal alcohol spectrum disorder

ROS:

Reactive oxygen species

LP:

Lipid peroxidation

PC:

Protein carbonyl content

CL:

Chemiluminescence assay

GSH:

Reduced glutathione

GP:

Glutathione peroxidase

GR:

Glutathione reductase

SOD:

Superoxide dismutase

CAT:

Catalase

MDA:

Malondialdehyde

NF-κB:

Nuclear factor kappa B

PIC:

Proinflammatory cytokine

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  1. Department of Medical Science Biology, Cerrahpasa Medical Faculty, Istanbul University—Cerrahpasa, 34096, Fatih/Istanbul, Turkey

    Erdal Ince

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Ince, E. The protective effect of quercetin in the alcohol-induced liver and lymphoid tissue injuries in newborns. Mol Biol Rep 47, 451–459 (2020). https://doi.org/10.1007/s11033-019-05148-0

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