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Functions, mechanisms and regulation of Pumilio/Puf family RNA binding proteins: a comprehensive review

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Abstract

The Pumilio (Pum)/Puf family proteins are ubiquitously present across eukaryotes, including yeast, plants and humans. They generally bind to the 3′ untranslated regions of single stranded RNA targets in a sequence specific manner and destabilize them, although a few reports suggest their role in stabilizing the target transcripts. The Pum isoforms are implicated in a wide array of biological processes including stem cell maintenance, development, ribosome biogenesis as well as human diseases. Further studies on Pum would be interesting and important to understand their evolutionarily conserved and divergent features across species, which can have potential implications in medicine, plant sciences as well as basic molecular and cell biological studies. A large number of research reports exists, pertaining to various aspects of Pum, in individual experimental systems. This review is a comprehensive summary of the functions, types, mechanism of action as well as the regulation of Pum in various species. Also, the research questions to be addressed in future are discussed.

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Acknowledgements

The research in the authors’ lab was funded by grants to BS, from Department of Science and Technology, Govt. of India (Grant Sanction Number: SB/YS/LS-188/2014) and Department of Biotechnology, Govt. of India (Grant Sanction Number: BT/Bio-CARe/02/10,078/2013–14). MJN receives and acknowledges the DST-INSPIRE Fellowship (IF170376) from Department of Science and Technology, Govt. of India. The authors are thankful to Mr. S A Sheshadri for critical reading and suggestions regarding the manuscript. The authors thank SASTRA Deemed to be University for the infrastructural facilities.

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    M. J. Nishanth & Bindu Simon

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Nishanth, M.J., Simon, B. Functions, mechanisms and regulation of Pumilio/Puf family RNA binding proteins: a comprehensive review. Mol Biol Rep 47, 785–807 (2020). https://doi.org/10.1007/s11033-019-05142-6

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