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Diagnostic accuracy of interferon (IFN)-γ inducible protein 10 (IP-10) as a biomarker for the discrimination of active and latent tuberculosis

Molecular Biology Reports volume 46, pages 6263–6269 (2019)Cite this article

Abstract

To assess the potency of Interferon (IFN)-γ inducible protein 10 (IP-10) stimulated by recombinant PE35 and PPE68 as a biomarker in differentiating between active and latent tuberculosis. Patients with active pulmonary TB (PTB) (n = 30), latent TB infection (LTBI) (n = 29), and BCG-vaccinated healthy controls (HCs) (n = 30) were enrolled and blood samples were taken from them. The diagnostic performance of IP-10 was evaluated by the Receiver operator characteristic (ROC) curve and the area under the curve (AUC) and their 95% confidence intervals (CI) were calculated. The median IP-10 concentrations following stimulation with recombinant PE35 and PPE68 were significantly higher in TB-infected group (both PTB and LTBI) compared with HCs (P < 0.05). It was also significantly higher in PTB patients compared with individuals with LTBI (P < 0.05). The discriminatory performance of IP-10 following stimulation with recombinant PE35 and PPE68 (assessed by AUC) between TB patients and HCs were similar (AUC: 0.79 [95% CI 0.68–0.89] and 0.79 [95% CI 0.69–0.89], respectively). AUCs of IP-10 following stimulation with recombinant PE35 and PPE68 for distinguishing between PTB and LTBI groups were 0.63 (95% CI 0.47–0.79) and 0.61 (0.45–0.77), respectively. Under the selected cut-off values, the sensitivity and specificity of IP-10 for distinguishing of TB-infected and HCs after stimulation with recombinant PE35 was 74.5% and 73%, respectively and after stimulation with recombinant PPE68 were 76.5% and 63%, respectively. Moreover, the sensitivity and specificity of IP-10 for differentiating of PTB and LTBI following stimulation with recombinant PE35 and PPE68 were 770 pg/ml (sensitivity: 63%; specificity: 62%) and 502 pg/ml (sensitivity: 80%; specificity: 52%), respectively. IP-10 stimulated by recombinant PE35 and PPE68 is a promising biomarker for TB diagnosis. However, it doesn’t have desirable sensitivity and specificity in distinguishing between PTB and LTBI.

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Funding

This work was supported by a grant from the Tehran University of Medical Sciences, Tehran, Iran with project Grant Number (94-04-159-31392).

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Authors and Affiliations

  1. Pediatric Infectious Disease Research Center, Children Medical Center Hospital, Tehran University of Medical Sciences, No.62, Gharib St., Keshavarz Blvd., Tehran, Iran

    Setareh Mamishi, Shima Mahmoudi, Maryam Banar, Reihaneh Hosseinpour Sadeghi & Babak Pourakbari

  2. Department of Infectious Diseases, Pediatrics Center of Excellence, Children’s Medical Center, Tehran University of Medical Sciences, Tehran, Iran

    Setareh Mamishi

  3. Clinical Tuberculosis and Epidemiology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Majid Marjani

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  1. Setareh Mamishi
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Correspondence to Babak Pourakbari.

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Mamishi, S., Mahmoudi, S., Banar, M. et al. Diagnostic accuracy of interferon (IFN)-γ inducible protein 10 (IP-10) as a biomarker for the discrimination of active and latent tuberculosis. Mol Biol Rep 46, 6263–6269 (2019). https://doi.org/10.1007/s11033-019-05067-0

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  • DOI: https://doi.org/10.1007/s11033-019-05067-0

Keywords

  • M. tuberculosis
  • IP-10
  • PE35
  • PPE68
  • Active tuberculosis
  • Latent tuberculosis