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Radioprotective effect of olanzapine as an anti-psychotic drug against genotoxicity and apoptosis induced by ionizing radiation on human lymphocytes

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Abstract

Olanzapine (OLA), is prescribed as an anti-psychotic medicine in schizophrenia patients. In this study, the protective effect of OLA against genotoxicity and apoptosis induced by ionizing radiation in human healthy lymphocytes was evaluated. At first, the antioxidant activities of OLA were assayed by two different methods as free radical scavenging with DPPH (2,2-diphenyl-1-picryl-hydrazyl) and ferric reducing power methods. In in vitro experiment, human blood samples were treated with OLA at various concentrations (0.25–20 μM) for 3 h and then were exposed to X-ray at a dose of 150 cGy. The genotoxicity was assessed in binucleated human lymphocytes with micronuclei assay. The apoptotic lymphocytes were assessed by flow cytometry in OLA treated and/or irradiated lymphocytes. OLA exhibited free radical scavenging and reducing power activities more than ascorbic acid. The results showed that the lymphocytes treated with OLA and later exposed to IR presented lower frequencies of micronuclei and apoptosis compared to the control sample which was irradiated and not treated to OLA. The maximum radioprotection was observed at 20 μM of OLA with 83% of efficacy. The present study suggested the protective role for OLA in protection radiation-induced genetic damage and apoptosis induced by ionizing irradiation in human normal cells.

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Funding

Funding was provided by Elite Researcher Grant Committee from the National Institute for Medical Research Development, Grant No. 963296, Tehran, Iran.

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Authors and Affiliations

  1. Department of Radiopharmacy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran

    Mohammad Asghari, Zahra Shaghaghi, Soghra Farzipour & Seyed Jalal Hosseinimehr

  2. Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran

    Mohammad Asghari

  3. Department of Nuclear Medicine and Molecular Imaging, Clinical Development Research Unit of Farshchian Heart Center, Hamadan University of Medical Sciences, Hamadan, Iran

    Zahra Shaghaghi

  4. Department of Radiology and Radiation Oncology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran

    Arash Ghasemi

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  1. Mohammad Asghari
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  2. Zahra Shaghaghi
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Correspondence to Seyed Jalal Hosseinimehr.

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This study was approved by research and ethical committees of the Iranian National Institute for Medical Research and Development (ID#IR.NIMAD.REC.1396. 235).

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Written informed consents were also obtained from healthy male volunteers. This study was performed only in vitro experiments on blood samples of healthy male volunteers.

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Asghari, M., Shaghaghi, Z., Farzipour, S. et al. Radioprotective effect of olanzapine as an anti-psychotic drug against genotoxicity and apoptosis induced by ionizing radiation on human lymphocytes. Mol Biol Rep 46, 5909–5917 (2019). https://doi.org/10.1007/s11033-019-05024-x

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