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Role of Th22 cells in Helicobacter pylori-related gastritis and peptic ulcer diseases

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A Correction to this article was published on 08 May 2022

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Abstract

Helicobacter pylori (H. pylori) has been shown to be one of the leading causes of peptic ulcer diseases (PUDs) and gastritis. T helper-22 (Th22) cells and its most important cytokine, interleukin-22 (IL-22) are importantly active in inflammation and inflammatory tissues. Since inflammation is one of the main attributes of infection caused by H. pylori and resulting complications (gastritis and gastrointestinal ulcer), this study was designed to evaluate the Th22 cells count and the IL-22 protein expression in people suffering from PUD and gastritis. The present study was conducted on 55 patients with gastritis, 47 patients with PUD and 48 uninfected subjects. After preparation of section and extraction of protein from antral biopsies, immunohistochemistry and western blot methods were used to evaluate the Th22 cells and IL-22 protein expression level, respectively. According to findings, the Th22 cells count and the IL-22 protein expression level in the infected subjects were siginficantly more than in the uninfected subjects. It should be noted that the Th22 cells count and the IL-22 protein expression level in the infected subjects with PUD were significantly greater than those in the infected subjects with gastritis. In addition, the Th22 cells count had positive correlation with the density of H. pylori, chronic inflammation score and acute inflammatory score in the infected subjects with PUD. The Th22 cells count had positive correlation with the Th17 cells count and inverse correlation with the Treg cells count in the infected subjects with PUD and gastritis. Our data demonstrated that abnormal hyper-activation of Th22 cells as well as its correlation with the Th17 cells during infection caused by H. pylori might damage tissues through immunopathological responses.

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Acknowledgements

The creators are appreciative to the Staffs of Students Research Committee, Shahrekord University of Medical Sciences, Shahrekord, Iran, and also to the Staffs of Cellular and Molecular Research Center, Shahrekord University of Medical Sciences and the Specialists of the Endoscopy Unit of Shahrekord Hajar Hospital for their participation.

Funding

The funding was provided by Research Deputy of Shahrekord University of Medical Sciences with Grant Number 1332.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran

    Ahmad Sanaii

  2. Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran

    Hedayatollah Shirzad & Nader Bagheri

  3. Department of Cellular and Molecular Biology, Azad University of Shahrekord, Shahrekord, Iran

    Mehrnoosh Haghighian

  4. Department of Internal Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran

    Ghorbanali Rahimian & Mohammadhadi Shafigh

  5. Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran

    Amin Soltani

  6. Department of Pathology, Shahrekord University of Medical Sciences, Shahrekord, Iran

    Kamran Tahmasbi

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  1. Ahmad Sanaii
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  2. Hedayatollah Shirzad
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  3. Mehrnoosh Haghighian
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  8. Nader Bagheri
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Correspondence to Nader Bagheri.

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The research was approved by the Ethical Board of Shahrekord University of Medical Sciences with Number IR.SKUMS.134.

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Sanaii, A., Shirzad, H., Haghighian, M. et al. Role of Th22 cells in Helicobacter pylori-related gastritis and peptic ulcer diseases. Mol Biol Rep 46, 5703–5712 (2019). https://doi.org/10.1007/s11033-019-05004-1

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