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Isolation and characterization of microsatellites loci in Spondias purpurea (Anacardiaceae) and cross amplification in congeneric species


Microsatellite markers provide high polymorphism levels, useful to study genetic diversity and gene flow patterns in plant populations. Here we develop and characterize microsatellite primers to evaluate patterns of genetic structure and diversity, and gene flow levels in the dioecious tropical tree Spondias purpurea (Anacardiaceae). Twenty-four microsatellite primers were developed for Spondias purpurea. Polymorphism was evaluated in 139 individuals from three localities in Mexico. Ten loci were polymorphic. The number of alleles ranged between two and 21, the average number of alleles was 5.88. Cross-amplification trials on S. mombin, S. radlkoferi, Astronium graveolens and Amphipterygium adstringens achieved successful amplification for only six microsatellites in S. mombin and S. radlkoferi. Microsatellites developed for S. purpurea will be a useful tool to estimate genetic diversity within and among populations, as well as to assess the consequences of habitat fragmentation on gene flow patterns of this species.

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  1. Miller A, Schaal B (2005) Domestication of a Mesoamerican cultivated fruit tree, Spondias purpurea. Proc Natl Acad Sci USA 102:12801–12806.

    CAS  Article  PubMed  Google Scholar 

  2. Miller A, Knouft J (2006) GIS-based characterization of the geographic distributions of wild and cultivated populations of the Mesoamerican fruit tree Spondias purpurea (Anacardiaceae). Am J Bot 93:1757–1767.

    Article  PubMed  Google Scholar 

  3. Mitchell JD, Daly DC (2015) A revision of Spondias L. (Anacardiaceae) in the Neotropics. PhytoKeys 55:1–92.

    Article  Google Scholar 

  4. Méndez-Toribio M, Pierro A, Quesada M, Benítez-Malvido J (2014) Regeneration beneath a dioecious tree species Spondias purpurea in a Mexican tropical dry forest. J Trop Ecol 30:265–268.

    Article  Google Scholar 

  5. Mandujano S, Gallina S, Bullock S (1994) Frugivory and dispersal of Spondias purpurea (Anacardiaceae) in a tropical deciduous forest in México. Rev Biol Trop 42:107–114

    Google Scholar 

  6. Miller A (2011) Spondias. In: Kole C (ed) Wild crop relatives: genomic and breeding resources. Springer, Berlin Heidelberg, pp 203–212

    Chapter  Google Scholar 

  7. Macía M, Barfod A (2000) Economic botany of Spondias purpurea (Anacardiaceae) in Ecuador. Econ Bot 54:449–458.

    Article  Google Scholar 

  8. Miller AJ, Gross BL (2011) From forest to field: perennial fruit crop domestication. Am J Bot 98:1389–1414.

    Article  PubMed  Google Scholar 

  9. Aguilar-Barajas E, Sork VL, González-Zamora A, Rocha-Ramírez V, Arroyo-Rodríguez V, Oyama K (2014) Isolation and characterization of polymorphic microsatellite loci in Spondias radlkoferi (Anacardiaceae). Appl Plant Sci 2(11):10.

    Article  Google Scholar 

  10. Arroyo-Rodríguez V, Aguilar-Barajas E, González-Zamora A, Rocha-Ramírez V, González-Rodríguez A, Oyama K (2017) Parent-parent and parent-offspring distances in Spondias radlkoferi seeds suggest long-distance pollen and seed dispersal: evidence from latrines of the spider monkey. J Tropical Ecol 33(2):95–106.

    Article  Google Scholar 

  11. Rozen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol 132:365–386

    CAS  PubMed  Google Scholar 

  12. Excoffier L, Laval G, Schneider S (2007) Arlequin (version 3.0): an integrated software package for population genetics data analysis. Evol Bioinform 1:47–50

    Google Scholar 

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This study was performed in partial fulfilment of the requirements of the Ph. D. degree of EJCP at the Graduate Program in Biological Sciences, UNAM. We thank Gumersindo Sanchez-Montoya for help in the Lab. This work was supported by grants from Universidad Nacional Autónoma de México (PAPIIT # IN212714-3, IV200418), SAGARPA-CONACYT 291333, CONACYT-UNAM-UAGro to LANASE (2015-LN250996, 2016-LN271449, 2017-LN280505, 2018-LN293701) and Programa Iberoamericana de Ciencia y Tecnologıa para el Desarrollo RED CYTED-SEPODI (417RT0527).

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Correspondence to Mauricio Quesada.

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Cristóbal-Pérez, E.J., Fuchs, E.J., Harvey, N. et al. Isolation and characterization of microsatellites loci in Spondias purpurea (Anacardiaceae) and cross amplification in congeneric species. Mol Biol Rep 46, 5581–5585 (2019).

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  • Anacardiaceae
  • Habitat fragmentation
  • Microsatellites
  • Spondias