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Isolation and characterization of microsatellites loci in Spondias purpurea (Anacardiaceae) and cross amplification in congeneric species

Abstract

Microsatellite markers provide high polymorphism levels, useful to study genetic diversity and gene flow patterns in plant populations. Here we develop and characterize microsatellite primers to evaluate patterns of genetic structure and diversity, and gene flow levels in the dioecious tropical tree Spondias purpurea (Anacardiaceae). Twenty-four microsatellite primers were developed for Spondias purpurea. Polymorphism was evaluated in 139 individuals from three localities in Mexico. Ten loci were polymorphic. The number of alleles ranged between two and 21, the average number of alleles was 5.88. Cross-amplification trials on S. mombin, S. radlkoferi, Astronium graveolens and Amphipterygium adstringens achieved successful amplification for only six microsatellites in S. mombin and S. radlkoferi. Microsatellites developed for S. purpurea will be a useful tool to estimate genetic diversity within and among populations, as well as to assess the consequences of habitat fragmentation on gene flow patterns of this species.

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Acknowledgements

This study was performed in partial fulfilment of the requirements of the Ph. D. degree of EJCP at the Graduate Program in Biological Sciences, UNAM. We thank Gumersindo Sanchez-Montoya for help in the Lab. This work was supported by grants from Universidad Nacional Autónoma de México (PAPIIT # IN212714-3, IV200418), SAGARPA-CONACYT 291333, CONACYT-UNAM-UAGro to LANASE (2015-LN250996, 2016-LN271449, 2017-LN280505, 2018-LN293701) and Programa Iberoamericana de Ciencia y Tecnologıa para el Desarrollo RED CYTED-SEPODI (417RT0527).

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Correspondence to Mauricio Quesada.

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Cristóbal-Pérez, E.J., Fuchs, E.J., Harvey, N. et al. Isolation and characterization of microsatellites loci in Spondias purpurea (Anacardiaceae) and cross amplification in congeneric species. Mol Biol Rep 46, 5581–5585 (2019). https://doi.org/10.1007/s11033-019-04968-4

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  • DOI: https://doi.org/10.1007/s11033-019-04968-4

Keywords

  • Anacardiaceae
  • Habitat fragmentation
  • Microsatellites
  • Spondias