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Effects of pH and sugar supplements on bacteriocin-like inhibitory substance production by Pediococcus pentosaceus

Molecular Biology Reports volume 46, pages 4883–4891 (2019)Cite this article

Abstract

To improve bacteriocin-like inhibitory substance (BLIS) production by Pediococcus pentosaceus ATCC 43200, the influence of pH as well as the addition of sugars—either prebiotic (inulin) or not (sucrose)—on its metabolism were investigated. This strain was grown at pH 5.0 or 6.0 either in glucose-based MRS medium (control) or after addition of 0.5, 1.0 or 1.5% (w/w) sucrose and inulin (GSI-MRS) in the same percentages. In the control medium at pH 5.0, cell mass concentration after 48 h of fermentation (Xmax = 2.26 g/L), maximum specific growth rate (µmax = 0.180 h−1) and generation time (Tg = 3.84 h) were statistically coincident with those obtained in supplemented media. At pH 6.0 some variations occurred in these parameters between the control medium (Xmax = 2.68 g/L; µmax = 0.32 h−1; Tg = 2.17 h) and the above supplemented media (Xmax = 1.90, 2.52 and 1.86 g/L; µmax = 0.26, 0.33 and 0.32 h−1; Tg = 2.62, 2.06 and 2.11 h, respectively). Lactate production was remarkable at both pH values (13 and 16 g/L) and improved in all supplemented media, being 34 and 54% higher than in their respective control media, regardless of the concentration of these ingredients. Cell-free supernatant of the fermented control medium at pH 5.0 displayed an antimicrobial activity against Enterococcus 101 5.3% higher than that at pH 6.0 and even 20% higher than those of all supplemented media, regardless of the concentration of supplements. BLIS production was favored either at pH 5.0 or in the absence of any additional supplements, which were able, instead, to stimulate growth and lactate production by P. pentosaceus.

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Funding

This study was funded by São Paulo Research Foundation (FAPESP) under Grants [2018/04385-8 and 2018/04563-3] and by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001 and the National Council for Scientific and Technological Development (CNPq). Collaboration of the Promotion of Scientific and Technological Development of CONICYT, FONDECYT / POSTDOCTORAL (N° 3180765) Chile.

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Authors and Affiliations

  1. Department of Biochemical and Pharmaceutical Technology Department, School of Pharmaceutical Sciences, University of São Paulo, Av. Lineu Prestes 580, São Paulo, 05508-900, Brazil

    Pamela Oliveira de Souza de Azevedo, Hernando Fernandes de Azevedo & Ricardo Pinheiro de Souza Oliveira

  2. Núcleo de Investigación en Producción Alimentaria. Departamento de Ciencias Biológicas y Químicas. Facultad de Recursos Naturales, Universidad Católica de Temuco, Temuco, Chile

    Elías Figueroa

  3. Department of Civil, Chemical and Environmental Engineering, Pole of Chemical Engineering, Via Opera Pia 15, 16145, Genoa, Italy

    Attilio Converti

  4. Department of Chemical Engineering, Faculty of Science, University of Vigo (Campus Ourense), As Lagoas s/n, 32004, Ourense, Spain

    José Manuel Domínguez

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  1. Pamela Oliveira de Souza de Azevedo
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de Souza de Azevedo, P.O., de Azevedo, H.F., Figueroa, E. et al. Effects of pH and sugar supplements on bacteriocin-like inhibitory substance production by Pediococcus pentosaceus. Mol Biol Rep 46, 4883–4891 (2019). https://doi.org/10.1007/s11033-019-04938-w

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