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Epidemiological characterization, genetic alterations of Helicobacter pylori infection in chronic gastric disorder and prognostic values of heterozygosity loss in chromosome 3p

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Abstract

From the literature review, there seem to be no studies conducted on infection caused by Helicobacter pylori in patients with gastric MALT lymphoma in the KSA region. The present research is an attempt to understand the prevalence of patients infected with H. pylori in the selected region and the role of allelic imbalance of chromosome 3p regions to understand the clinical manifestations and features associated with MALT lymphomagenesis. The researcher analyzed the frequency of infection in patients from the region of Saudi Arabia by examining the data collected from hospitals and biopsy tissue samples as per the recommended protocol. The endoscopic diagnosis was performed to collect biopsy samples. Histology and AP-PCR DNA fingerprinting analyses were performed from the endoscopic gastric mucosal biopsies collected from patients with associated gastric MALT lymphoma. The existence of H. pylori was examined based on the results of gastric mucosal biopsies stained with hematoxylin–eosin (H&E) and Steiner’s silver stains. MALT, MALT lymphoma tissue samples and H. pylori-positive chronic gastritis were examined for LOH at chromosome 3p24 using standard procedures and techniques. The findings of the paper revealed the H. pylori was found to be positive in 17% of the cases significantly high among the age group of 31–50 years. Patients with MALT, MALT lymphoma, and H. pylori-associated gastritis presented features such as lymphocyte accumulation, vacuolation, Peyer’s patch appearance, and lymphatic follicles. H. pylori were found to appear as a dense colored accumulated mass in the gastric epithelial layer. The findings from AP-PCR generated DNA fingerprints revealed intense band including two prominent bands in MALT lymphoma. Among other loci, 3p24 was the only one locus that showed high percentages of LOH as reported earlier in all cancer-related cases. The findings of this research paper empower the fact that allelic imbalances play a vital role in the development of MALT lymphoma. However, future researches should be conducted to identify the chromosome regions of the AP-PCR generated DNA fingerprints of human gastric MALT lymphoma in order to confirm this proposition.

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References

  1. Lee AD, Fox J, Hazell S (1993) Pathogenicity of Helicobacter pylori: a perspective. Infect Immun 61(5):1601

    CAS  PubMed  PubMed Central  Google Scholar 

  2. Mobley HL (1997) Helicobacter pylori factors associated with disease development. Gastroenterology 113(6):S21–28

    Article  CAS  PubMed  Google Scholar 

  3. Uemura N, Okamoto S, Yamamoto S, Matsumura N, Yamaguchi S, Yamakido M, Taniyama K, Sasaki N, Schlemper RJ (2001) Helicobacter pylori infection and the development of gastric cancer. N Engl J Med 345(11):784–789

    Article  CAS  PubMed  Google Scholar 

  4. Makola D, Peura DA, Crowe SE (2007) Helicobacter pylori infection and related gastrointestinal diseases. J Clin Gastroenterol 41(6):548–558

    Article  PubMed  Google Scholar 

  5. Chan AO, Chu KM, Yuen ST, Leung SY, Lam SK, Wong J (2001) Synchronous gastric adenocarcinoma and mucosa-associated lymphoid tissue lymphoma in association with Helicobacter pylori infection: comparing reported cases between the East and West. Am J Gastroenterol 96(6):1922–1924

    Article  CAS  PubMed  Google Scholar 

  6. Lindkvist P, Asrat D, Nilsson I, Tsega E, Olsson GL, Wretlind B, Giesecke J (1996) Age at acquisition of Helicobacter pylori infection: comparison of a high and a low prevalence country. Scand J Infect Dis 28(2):181–184

    Article  CAS  PubMed  Google Scholar 

  7. Bardhan PK (1997) Epidemiological features of Helicobacter pylori infection in developing countries. Clin Infect Dis 25(5):973–978

    Article  CAS  PubMed  Google Scholar 

  8. Allum WH (2013) Epidemiology, genetics and screening for oesophageal and gastric cancer. In: MichaelGriffin S, Lamb P (eds) Oesophagogastric Surgery E-Book: Companion to Specialist Surgical Practice. Elsevier, Amsterdam, p 22

    Google Scholar 

  9. Chomvarin C, Namwat W, Chaicumpar K, Mairiang P, Sangchan A, Sripa B, Tor-Udom S, Vilaichone RK (2008) Prevalence of Helicobacter pylori vacA, cagA, cagE, iceA and babA2 genotypes in Thai dyspeptic patients. Int J Infect Dis 12(1):30–36

    Article  CAS  PubMed  Google Scholar 

  10. Sagaert X, Van Cutsem E, De Hertogh G, Geboes K, Tousseyn T (2010) Gastric MALT lymphoma: a model of chronic inflammation-induced tumor development. Nat Rev Gastroenterol Hepatol 7:336–346

    Article  CAS  PubMed  Google Scholar 

  11. Kim DY, Kim YS, Huh HJ, Choi JS, Yeo JS, Kwak BS, Chae SL (2011) A case of monoclonal gammopathy in extranodal marginal zone B-cell lymphoma of the small intestine. Korean J Lab Med 31:18–21

    Article  PubMed  PubMed Central  Google Scholar 

  12. Kusters JG, van Vliet AH, Kuipers EJ (2006) Pathogenesis of Helicobacter pylori infection. Clin Microbiol Rev 19(3):449–490

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Isaacson PG, Du MQ (2004) MALT lymphoma: from morphology to molecules. Nat Rev Cancer 4(8):644

    Article  CAS  PubMed  Google Scholar 

  14. Cavalli F, Isaacson PG, Gascoyne RD, Zucca E (2001) MALT lymphomas. ASH Educ Program Book 2001(1):241–258

    Google Scholar 

  15. Gossage L, Eisen T, Maher ER (2015) VHL, the story of a tumour suppressor gene. Nat Rev Cancer 15(1):55

    Article  CAS  PubMed  Google Scholar 

  16. Lasko D, Cavenee W, Nordenskjöld M (1991) Loss of constitutional heterozygosity in human cancer. Annu Rev Genet 25(1):281–314

    Article  CAS  PubMed  Google Scholar 

  17. Banerji S, Cibulskis K, Rangel-Escareno C, Brown KK, Carter SL, Frederick AM, Lawrence MS, Sivachenko AY, Sougnez C, Zou L, Cortes ML (2012) Sequence analysis of mutations and translocations across breast cancer subtypes. Nature 486(7403):405

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Dixon MF (1994) Pathophysiology of Helicobacter pylori infection. Scand J Gastroenterol 29(suppl 201):7–10

    Article  Google Scholar 

  19. Padilla PI, Wada A, Yahiro K, Kimura M, Niidome T, Aoyagi H, Kumatori A, Anami M, Hayashi T, Fujisawa JI, Saito H (2000) Morphologic differentiation of HL-60 Cells is associated with appearance of RPTPβ and induction of Helicobacter pylori VacA sensitivity. J Biol Chem 275(20):15200–15206

    Article  CAS  PubMed  Google Scholar 

  20. Sagaert X, De Wolf-Peeters C, Noels H, Baens M (2007) The pathogenesis of MALT lymphomas: where do we stand? Leukemia 21(3):389

    Article  CAS  PubMed  Google Scholar 

  21. Salim EI, Moore MA, Al-Lawati JA, Al-Sayyad J, Bazawir A, Bener A, Corbex M, El-Saghir N, Habib OS, Maziak W, Mokhtar HC (2009) Cancer epidemiology and control in the arab world-past, present and future. Asian Pac J Cancer Prev 10(1):3–16

    PubMed  Google Scholar 

  22. Gatta L, Vakil N, Vaira D, Scarpignato C (2013) Global eradication rates for Helicobacter pylori infection: systematic review and meta-analysis of sequential therapy. BMJ 7(347):f4587

    Article  Google Scholar 

  23. Lengauer C, Kinzler KW, Vogelstein B (1998) Genetic instabilities in human cancers. Nature 396(6712):643

    Article  CAS  PubMed  Google Scholar 

  24. Lord CJ, Ashworth A (2012) The DNA damage response and cancer therapy. Nature 481(7381):287

    Article  CAS  PubMed  Google Scholar 

  25. Rajagopalan H, Nowak MA, Vogelstein B, Lengauer C (2003) The significance of unstable chromosomes in colorectal cancer. Nat Rev Cancer 3(9):695

    Article  CAS  PubMed  Google Scholar 

  26. Klein CA, Schmidt-Kittler O, Schardt JA, Pantel K, Speicher MR, Riethmüller G (1999) Comparative genomic hybridization, loss of heterozygosity, and DNA sequence analysis of single cells. Proc Natl Acad Sci 96(8):4494–4499

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Charames GS, Bapat B (2003) Genomic instability and cancer. Curr Mol Med 3(7):589–596

    Article  CAS  PubMed  Google Scholar 

  28. Goode EL, Ulrich CM, Potter JD (2002) Polymorphisms in DNA repair genes and associations with cancer risk. Cancer Epidemiol Prev Biomark 11(12):1513–1530

    CAS  Google Scholar 

  29. Deng G, Lu Y, Zlotnikov G, Thor AD, Smith HS (1996) Loss of heterozygosity in normal tissue adjacent to breast carcinomas. Science 274(5295):2057–2059

    Article  CAS  PubMed  Google Scholar 

  30. Sagaert X. Helicobacter pylori Infection and MALT Lymphoma. In Helicobacter pylori Research 2016 (pp. 423-441). Springer, Tokyo

  31. Wotherspoon AC, Finn TM, Isaacson PG (1995) Trisomy 3 in low-grade B-cell lymphomas of mucosa-associated lymphoid tissue. Blood 85(8):2000–2004

    Article  CAS  PubMed  Google Scholar 

  32. Ott G, Kalla J, Steinhoff A, Rosenwald A, Katzenberger T, Roblick U, Ott MM, Müller-Hermelink HK (1998) Trisomy 3 is not a common feature in malignant lymphomas of mucosa-associated lymphoid tissue type. Am J Pathol 153(3):689–694

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  33. Oberstraß JÜ, Reifenberger G, Reifenberger J, Wechsler W, Collins VP (1996) Mutation of the Von Hippel-Lindau tumour suppressor gene in capillary haemangioblastomas of the central nervous system. J Pathol 179(2):151–156

    Article  PubMed  Google Scholar 

  34. Du M, Peng H, Singh N, Isaacson PG, Pan L (1995) The accumulation of p53 abnormalities is associated with progression of mucosa-associated lymphoid tissue lymphoma. Blood 86(12):4587–4593

    Article  CAS  PubMed  Google Scholar 

  35. Dyer MJ, Siebert R (2010) Chromosomal and molecular alterations in lymphoid malignancies: basic principles and detection methods. The Lymphoid Neoplasms 3ed. CRC Press, London, pp 332–351

    Google Scholar 

Download references

Acknowledgements

This project was funded and supported by the Research Groups Program (Research Group number RG-1440-070), Deanship of Scientific Research, King Saud University, Riyadh, Saudi Arabia.

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Authors and Affiliations

  1. Department of Basic Medical Science, College of Medicine, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia

    Abdul wahab Ali Abuderman

  2. Department of Pharmaceutics, College of Pharmacy, King Saud University, PO Box 2457, Riyadh, 11451, Saudi Arabia

    Rabbani Syed

  3. Department of Clinical Laboratory Science, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia

    Ayesha Mateen & Abdul Malik

  4. Department of Biochemistry, College of Science, King Saud University, PO Box 2455, Riyadh, Saudi Arabia

    Mohammad Shamsul Ola

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  1. Abdul wahab Ali Abuderman
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  2. Rabbani Syed
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  3. Ayesha Mateen
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  5. Mohammad Shamsul Ola
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Correspondence to Rabbani Syed.

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Abuderman, A.A., Syed, R., Mateen, A. et al. Epidemiological characterization, genetic alterations of Helicobacter pylori infection in chronic gastric disorder and prognostic values of heterozygosity loss in chromosome 3p. Mol Biol Rep 46, 4323–4332 (2019). https://doi.org/10.1007/s11033-019-04886-5

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  • DOI: https://doi.org/10.1007/s11033-019-04886-5

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