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Implantation associated divergence in neutrophil glucocorticoid and cysteine-X-cysteine receptor genes in cattle

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Abstract

The differential roles of Interferon-tau (IFNT) and Progesterone (P4) in triggering pro- and anti-inflammatory responses during peri-implantation period were assessed in cows. An ex vivo study was done by isolating neutrophils from the animals post artificial insemination (AI) at defined intervals and analyzing the expression changes of Glucocorticoid receptor-α (GRα) and Interleukin-8 receptor (CXCR1) genes. An in vitro study was also done isolating neutrophils from prepubertal heifers subjecting them to IFNT and Progesterone P4 supplementation separately. We observed that from day 14 post Artificial Insemination, there is a significant up-regulation of neutrophil GRα and CXCR1 in pregnant and non-pregnant cows respectively. The in vitro study showed that IFNT significantly up-regulates GRα whereas P4 significantly up-regulates CXCR1. The total leukocyte count and neutrophil count were also significantly higher in non-pregnant cows. We conclude that IFNT efficiently mediates neutrophil immunosuppression during peri-implantation period in cows by interacting with Interleukin 8 receptor and can also exert ligand independent actions on GRα.

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Acknowledgements

The authors acknowledge the help of all technical and non-technical staff at National Dairy Research Institute, Karnal for assistance in sample collection.

Funding

This work was supported by the Department of Biotechnology [Grant No. BT/PR8404/AAQ/1/548/2013; DATED: 20/06/2014], Ministry of Science and Technology, Government of India which has also awarded INSPIRE fellowship.

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Authors and Affiliations

  1. Krishi Vigyan Kendra, KVK Venkatagiri (YSR Horticulture University), Nellore, Andhra Pradesh, 524132, India

    P. Manjari

  2. Department of Veterinary Physiology, NTR CVSc, Gannavaram, 521102, India

    Iqbal Hyder

  3. Institute of Farm Animal Genetics (FLI), Mariensee, 31535, Neustadt, Germany

    Iqbal Hyder

  4. Division of Dairy Cattle Physiology, ICAR-NDRI, Karnal, Haryana, 132001, India

    A. K. Dang

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  1. P. Manjari
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  2. Iqbal Hyder
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  3. A. K. Dang
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Contributions

PM performed the study, conducted experiments and analyzed the data, IH drafted and edited the manuscript, AKD conception and design of the study.

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Correspondence to P. Manjari.

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The authors declared that they have no conflict of interest.

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Manjari, P., Hyder, I. & Dang, A.K. Implantation associated divergence in neutrophil glucocorticoid and cysteine-X-cysteine receptor genes in cattle. Mol Biol Rep 46, 5493–5499 (2019). https://doi.org/10.1007/s11033-019-04865-w

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  • DOI: https://doi.org/10.1007/s11033-019-04865-w

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