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Expression analysis and genotyping of DGKZ: a GWAS-derived risk gene for schizophrenia

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Abstract

Schizophrenia (SCZ) is a disabling and severe mental illness characterized by abnormal social behavior and disrupted emotions. Similar to other neuropsychological disorders, both genetics and environmental factors interplay so as to develop SCZ. It is acknowledged that genes such as DGKZ are involved in lipid signaling pathways that are the basis of neural activities, memory, and learning and are considered as candidate loci for SCZ. The aim of the present study was to evaluate the expression level and genotypes of DGKZ in patients with SCZ and controls. We used q-PCR to measure the relative expression of DGKZ in blood. To determine DGKZ–rs7951870 genotypes, tetra-ARMS PCR was used. Our results showed a significant difference in DGKZ mRNA ratio between SCZ patients and healthy controls (P = 2 × 10−4). Also, we showed that rs7951870-TT genotype was strongly associated with increased DGKZ expression level (P = 0.038). In conclusion, our findings revealed dysregulation of DGKZ in SCZ patients and a significant correction between the gene expression and DGKZ variant rs7951870.

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Acknowledgement

The present work was partially supported by Shahid Beheshti University of Medical Sciences (Tehran, Iran) and Semnan University of Medical Sciences (Semnan, Iran).

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Authors and Affiliations

  1. Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Somayeh Alinaghi, Amir Hossein Johari & Hamid Ghaedi

  2. Student Research Committee, Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Elham Alehabib

  3. The Cohort Lab for the Iran University of Medical Sciences Staffs, University of Medical Sciences, Tehran, Iran

    Fatemeh Vafaei & Shima Salehi

  4. Cancer Research Center, Semnan University of Medical Sciences, Semnan, Iran

    Hossein Darvish

  5. Department of Medical Genetics, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran

    Hossein Darvish

Authors
  1. Somayeh Alinaghi
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  2. Elham Alehabib
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  3. Amir Hossein Johari
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  4. Fatemeh Vafaei
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  5. Shima Salehi
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Correspondence to Hossein Darvish or Hamid Ghaedi.

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Alinaghi, S., Alehabib, E., Johari, A.H. et al. Expression analysis and genotyping of DGKZ: a GWAS-derived risk gene for schizophrenia. Mol Biol Rep 46, 4105–4111 (2019). https://doi.org/10.1007/s11033-019-04860-1

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  • DOI: https://doi.org/10.1007/s11033-019-04860-1

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