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Cytotoxicity screening of supercritical fluid extracted seaweeds and phenylpropanoids

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Abstract

Detached leaves of Posidonia oceanica and Zostera marina creating nuisance at the shores were extracted by means of supercritical CO2 enriched with a co-solvent, compared with that of soxhlet extraction. The extracts and their active compounds which are phenylpropanoids (chicoric, p-coumaric, rosmarinic, benzoic, ferulic and caffeic acids) were screened for cytotoxicity in cancer cell lines including human breast adenocarcinoma (MCF-7, MDA-MB-231, SK-BR-3), human colon adenocarcinoma (HT-29), human cervix adenocarcinoma (HeLa), human prostate adenocarcinoma (PC-3), Mus musculus neuroblastoma (Neuro 2A) cell lines and African green monkey kidney (VERO) as healthy cell line. Supercritical CO2 extracts proved to be more active than soxhlet counterparts. Particularly, Zostera marina extract obtained by supercritical CO2 at 250 bar, 80 °C, 20% co-solvent and a total flow rate of 15 g/min revealed the best IC50 values of 25, 20, 8 μg/ml in neuroblastoma, colon and cervix cancer cell lines. Among the major compounds tested, p-coumaric acid exhibited the highest cytotoxic against colon and cervix cell lines by with IC50 values of 25, 11 μg/ml. As for the effects on healthy cells, the extract was not cytotoxic indicating a selective cytotoxicity. Obtained supercritical CO2 extracts can be utilized as a supplement for preventive purposes.

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Acknowledgements

The financial support provided by the Research Fund of Kocaeli University (KOU-BAP 2013/022) and Türkiye Bilimsel ve Teknolojik Araştirma Kurumu (110M790) are appreciated. Additionally, authors are thankful to Dr. Halim Aytekin Ergul for providing the seaweed samples.

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Authors and Affiliations

  1. Department of Biology, Biotechnology Discipline, Science and Art Faculty, Kocaeli University, 41380, Izmit, Kocaeli, Turkey

    Canan Sevimli-Gur

  2. Department of Bioengineering, Faculty of Engineering, Ege University, 35100, Bornova, Izmir, Turkey

    Ozlem Yesil-Celiktas

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  1. Canan Sevimli-Gur
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Correspondence to Ozlem Yesil-Celiktas.

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Sevimli-Gur, C., Yesil-Celiktas, O. Cytotoxicity screening of supercritical fluid extracted seaweeds and phenylpropanoids. Mol Biol Rep 46, 3691–3699 (2019). https://doi.org/10.1007/s11033-019-04812-9

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  • DOI: https://doi.org/10.1007/s11033-019-04812-9

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