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Transcriptome sequencing of Eospalax fontanierii to determine hypoxia regulation of cardiac fibrinogen

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Abstract

With the increase in blood viscosity, the blood circulation resistance will increase when animals are in hypoxia. However, these phenomenons do not appear in hypoxic-adapted animals. Eospalax fontanierii is a subterranean rodent and is an ideal species for research in hypoxia adaptation. Eighteen healthy adult E. fontanierii individuals were equally divided into three groups that were exposed to 21% O2 for 1 week, 10.5% O2 for 44 h, and 6.5% O2 for 6 h, and then, the hearts were collected for transcriptome sequencing. After differentially expressed analysis, fibrinogen genes were selected for qPCR and Western blot verification. Eighteen healthy adult Sprague-Dawley rats (SD rats) were treated with the same oxygen concentrations, and their hearts were simultaneously subjected to qPCR. The quantitative real-time PCR and Western blot results were completely opposite to those of the rats. E. fontanierii fibrinogen mRNA was significantly downregulated when expressed under the conditions of 10.5% and 6.5% O2 compared with 21% O2. Correspondingly, fibrinogen mRNA in E. fontanierii was expressed at lower levels than SD rats in 10.5% and 6.5% O2. After tail-cutting experiment, the results showed that the coagulation rate of E. fontanierii was slowed down under hypoxic conditions. These results showed that E. fontanierii may downregulate the expression of fibrinogen mRNA in hypoxia to reduce the aggregation of red blood cells and platelets in plasma, which may prevent blood from becoming overly viscous, and at the same time, reduce blood circulation resistance and the probability of thrombosis in hypoxia to protect the heart.

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Data availability

The raw data and processed files of this article are available in the NCBI Gene Expression Omnibus with GEO Series Accession Number PRJNA497961.

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Acknowledgements

This study was supported by Grants from the National Nature Science Foundation of China (Grant Nos. 30670360 and 31770333) and Nature Science Foundation of Shaanxi Province, China (No. 2016JM3024).

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  1. Lulu Xu, Zhiqiang Hao, and Jinyan Lin contributed equally to this work.

Authors and Affiliations

  1. College of Life Science, Shaanxi Normal University, Xi’an, China

    Lulu Xu, Zhiqiang Hao, Jinyan Lin, Qiong Zhao, Baojun Zhang, Guanglin Li, Jianping He & Jingang Li

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  1. Lulu Xu
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  2. Zhiqiang Hao
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  3. Jinyan Lin
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  4. Qiong Zhao
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Contributions

JG Li and JP He conceived and designed the study. JY Lin and Q Zhao conducted most of the experiments with the help of LL Xu, GL Li, ZQ Hao and JP He provided regular timely suggestions during the course of the experimental work. LL Xu and JY Lin participated in several of the experiments. Q Zhao also helped LL Xu in preparing the first draft of the manuscript, which was edited and finalized by LL Xu and ZQ Hao with the help of GL Li, JP He, and JG Li. BJ Zhang provided the experimental materials.

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Correspondence to Jianping He or Jingang Li.

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All the animals were treated humanely according to guidelines of the Care and Use of Laboratory Animals of China, and all the procedures were approved by the Animal Care and Use Committee of Shaanxi Normal University.

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Xu, L., Hao, Z., Lin, J. et al. Transcriptome sequencing of Eospalax fontanierii to determine hypoxia regulation of cardiac fibrinogen. Mol Biol Rep 46, 5671–5683 (2019). https://doi.org/10.1007/s11033-019-04690-1

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