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Effects of dietary Allium mongolicum Regel polysaccharide on growth, lipopolysaccharide-induced antioxidant responses and immune responses in Channa argus

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Abstract

The present study evaluated the effects of dietary Allium mongolicum Regel polysaccharide (AMRP) on growth, lipopolysaccharide-induced antioxidant responses and immune responses in Channa argus. A basal diet was supplemented with AMRP at 0, 1, 1.5 or 2 g/kg feed for 56 days. After the 56 days feeding period, weight gain (WG), specific growth rate (SGR) and feed conversion ratio (FCR) were significantly increased or decreased (P < 0.05) by dietary AMRP, with the highest WG, SGR and the minimum FCR occurring in 1.5 g/kg AMRP group. Furthermore, AMRP supplementation conferred significant protective effects against LPS challenge by preventing alterations in the levels of complements 3 (C3) and complements 4 (C4), lysozyme, superoxide dismutase (SOD), glutathione-S-transferase (GST), interleukin-1β (IL-1β) and tumour necrosis factor-α (TNF-α) while regulating the expression of immune-related genes including heat shock protein 70 (HSP70), heat shock protein 90 (HSP90), SOD, GST, IL-1 and TNF-α. Finally, AMRP supplementation significantly increased serum total protein, albumin and globulin concentrations and reduced mortality after LPS challenge. Taken together, our results suggest that the administration of AMRP could attenuate LPS-induced negative effects in C. argus, with 1.5 g/kg considered a suitable dose.

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References

  1. Sagada G, Chen J, Shen B, Huang A, Sun L, Jiang J (2017) Optimizing protein and lipid levels in practical diet for juvenile northern snakehead fish (Channa argus). Anim Nutr 3:156–163

    Article  PubMed  PubMed Central  Google Scholar 

  2. Gou C, Wang J, Wang Y, Dong W, Shan X, Lou Y (2018) Hericium caput-medusae (Bull.:Fr.) Pers. polysaccharide enhance innate immune response, immune-related genes expression and disease resistance against Aeromonas hydrophila in grass carp (Ctenopharyngodon idella). Fish Shellfish Immunol 72:604–610

    Article  CAS  PubMed  Google Scholar 

  3. Cabello FC (2006) Heavy use of prophylactic antibiotics in aquaculture: a growing problem for human and animal health and for the environment. Environ Microbiol 8:1137–1144

    Article  CAS  PubMed  Google Scholar 

  4. Liu X, Steele JC, Meng XZ (2017) Usage, residue, and human health risk of antibiotics in Chinese aquaculture: a review. Environ Pollut 223:161–169

    Article  CAS  PubMed  Google Scholar 

  5. Bu Q, Wang B, Huang J, Liu K, Deng S, Wang Y (2016) Estimating the use of antibiotics for humans across China. Chemosphere 144:1384–1392

    Article  CAS  PubMed  Google Scholar 

  6. Kaleeswaran B, Ilavenil S, Ravikumar S (2012) Changes in biochemical, histological and specific immune parameters in Catla catla by Cynodon dactylon. J King Saud Univ Sci 24:139–152

    Article  Google Scholar 

  7. Herczeg D, Sipos D, Dán Á, Loy C, Kallert DM, Eszterbauer E (2017) The effect of dietary immunostimulants on the susceptibility of common carp (Cyprinus carpio) to the white spot parasite, Ichthyophthirius multifiliis. Parasitol Res 65:517–530

    CAS  Google Scholar 

  8. Chen R, Lochmann R, Goodwin A, Praveen K, Dabrowski K, Lee KJ (2004) Effects of dietary vitamins C and E on alternative complement activity, hematology, tissue composition, vitamin concentrations and response to heat stress in juvenile golden shiner (Notemigonus crysoleucas). Aquaculture 242:553–569

    Article  CAS  Google Scholar 

  9. Liu F, Shi HZ, Guo QS, Yu YB, Wang AM, Lv F (2016) Effects of astaxanthin and emodin on the growth, stress resistance and disease resistance of yellow catfish (Pelteobagrus fulvidraco). Fish Shellfish Immunol 51:125–135

    Article  CAS  PubMed  Google Scholar 

  10. Li J, Shi B, Yan S, Jin L, Guo Y, Li T (2013) Effects of dietary supplementation of chitosan on humoral and cellular immune function in weaned piglets. Anim Feed Sci Technol 186:204–208

    Article  CAS  Google Scholar 

  11. Jagruthi C, Yogeshwari G, Anbazahan SM, Mari LS Arockiaraj J, Mariappan P (2014) Effect of dietary astaxanthin against Aeromonas hydrophila infection in common carp, Cyprinus carpio. Fish Shellfish Immunol 41:674–680

    Article  CAS  PubMed  Google Scholar 

  12. Chew BP, Mathison BD, Hayek MG, Massimino S, Reinhart GA, Park JS (2011) Dietary astaxanthin enhances immune response in dogs. Vet Immunol Immunopathol 140:199–206

    Article  CAS  PubMed  Google Scholar 

  13. Wang E, Chen X, Wang K, Wang J, Chen D, Geng Y (2016) Plant polysaccharides used as immunostimulants enhance innate immune response and disease resistance against Aeromonas hydrophila infection in fish. Fish Shellfish Immunol 59:196–202

    Article  CAS  PubMed  Google Scholar 

  14. Qi S, Wang T, Chen R, Wang C, Ao C (2017) Effects of flavonoids from Allium mongolicum Regel on growth performance and growth-related hormones in meat sheep. Anim Nutr 3:33–38

    Article  PubMed  PubMed Central  Google Scholar 

  15. Qier MU, Changjin AO, Ruli SA, Wang T, Chen R, Muqile TE (2016) Effects of flavonoids from Allium mongolicum Regel on antioxidant capacity of meat sheep. Chin J Anima Nutr 4:46–54

    Google Scholar 

  16. Li M, Zhu X, Tian J, Liu M, Wang G (2019) Dietary flavonoids from Allium mongolicum Regel promotes growth, improves immune, antioxidant status, immune-related signaling molecules and disease resistance in juvenile northern snakehead fish (Channa argus). Aquaculture 501:473–481

    Article  CAS  Google Scholar 

  17. Zahran E, Risha E, Abdelhamid F, Mahgoub HA, Ibrahim T (2014) Effects of dietary Astragalus polysaccharides (APS) on growth performance, immunological parameters, digestive enzymes, and intestinal morphology of Nile tilapia (Oreochromis niloticus). Fish Shellfish Immunol 38:149–157

    Article  CAS  PubMed  Google Scholar 

  18. Zhou C, Lin H, Ge X, Niu J, Wang J, Wang Y (2015) The effects of dietary soybean isoflavones on growth, innate immune responses, hepatic antioxidant abilities and disease resistance of juvenile golden pompano Trachinotus ovatus. Fish Shellfish Immunol 43:158–166

    Article  CAS  PubMed  Google Scholar 

  19. Jiang J, Yin L, Li JY, Li Q, Shi D, Feng L (2017) Glutamate attenuates lipopolysaccharide -induced oxidative damage and mRNA expression changes of tight junction and defensin proteins, inflammatory and apoptosis response signaling molecules in the intestine of fish. Fish Shellfish Immunol 70:473–484

    Article  CAS  PubMed  Google Scholar 

  20. Bairwa MK, Jakhar JK, Reddy AD (2012) Animal and plant originated immunostimulants used in aquaculture. J Nat Prod Plant Resour 3:397–400

    Google Scholar 

  21. Hai NV (2015) The use of medicinal plants as immunostimulants in aquaculture: a review. Aquaculture 446:88–96

    Article  CAS  Google Scholar 

  22. Wang TT, Sun YX, Jin L, Xu YP, Li W, Ren TJ (2009) Enhancement of non-specific immune response in sea cucumber (Apostichopus japonicus) by Astragalus membranaceus and its polysaccharides. Fish Shellfish Immunol 27:757–762

    Article  CAS  PubMed  Google Scholar 

  23. Giri SS, Sen SS, Chi C, Kim HJ, Yun S, Park SC (2015) Chlorophytum borivilianum polysaccharide fraction provokes the immune function and disease resistance of labeo rohita against Aeromonas hydrophila. J Immunol Res 6:46–55

    Google Scholar 

  24. Yang X, Guo JL, Ye JY, Zhang YX, Wang W (2015) The effects of Ficus carica polysaccharide on immune response and expression of some immune-related genes in grass carp, Ctenopharyngodon idella. Fish Shellfish Immunol 42:132–147

    Article  CAS  PubMed  Google Scholar 

  25. Magnadóttir B (2006) Innate immunity of fish (overview). Fish Shellfish Immunol 20:137–145

    Article  CAS  PubMed  Google Scholar 

  26. Wu ZX, Pang SF, Liu JJ, Zhang Q, Fu SS, Du J (2013) Coriolus versicolor polysaccharides enhance the immune response of crucian carp (Corassius auratus gibelio) and protect against Aeromonas hydrophila. J Appl Ichthyol 29:562–578

    Article  CAS  Google Scholar 

  27. Ichiki S, Kato-Unoki Y, Somamoto T, Nakao M (2012) The binding spectra of carp C3 isotypes against natural targets independent of the binding specificity of their thioester. Dev Comp Immunol 38:10–26

    Article  CAS  PubMed  Google Scholar 

  28. Boshra H, Li J, Sunyer JO (2006) Recent advances on the complement system of teleost fish. Fish Shellfish Immunol 20:239–262

    Article  CAS  PubMed  Google Scholar 

  29. Kim JH, Kang JC (2015) Oxidative stress, neurotoxicity, and non-specific immune responses in juvenile red sea bream, Pagrus major, exposed to different waterborne selenium concentrations. Chemosphere 135:46–52

    Article  CAS  PubMed  Google Scholar 

  30. Raingeaud J, Gupta S, Rogers JS, Dickens M, Han J, Ulevitch RJ (1995) Pro-inflammatory cytokines and environmental stress cause p38 mitogen-activated protein kinase activation by dual phosphorylation on tyrosine and threonine. J Biol Chem 270:7420–7426

    Article  CAS  PubMed  Google Scholar 

  31. Umnova NV, Moskaleva EY, Poroshenko GG (1986) Role of DNA repair processes in sister chromatid exchange frequency changes in peripheral blood lymphocytes during inflammatory diseases. Bull Exp Biol Med 102:1748–1750

    Article  Google Scholar 

  32. Feng L, Chen YP, Jiang WD, Liu Y, Jiang J, Wu P (2016) Modulation of immune response, physical barrier and related signaling factors in the gills of juvenile grass carp (Ctenopharyngodon idella) fed supplemented diet with phospholipids. Fish Shellfish Immunol 48:79–93

    Article  CAS  PubMed  Google Scholar 

  33. Guo YL, Wu P, Jiang WD, Liu Y, Kuang SY, Jiang J (2018) The impaired immune function and structural integrity by dietary iron deficiency or excess in gill of fish after infection with Flavobacterium columnare: regulation of NF-κB, TOR, JNK, p38MAPK, Nrf2 and MLCK signalling. Fish Shellfish Immunol 74:593–608

    Article  CAS  PubMed  Google Scholar 

  34. Xu C, Li E, Suo Y, Su Y, Lu M, Zhao Q (2018) Histological and transcriptomic responses of two immune organs, the spleen and head kidney, in Nile tilapia (Oreochromis niloticus) to long-term hypersaline stress. Fish Shellfish Immunol 6:69–76

    Google Scholar 

  35. Li C, Ma D, Chen M, Zhang L, Zhang L, Zhang J (2016) Ulinastatin attenuates LPS-induced human endothelial cells oxidative damage through suppressing JNK/c-Jun signaling pathway. Biochem Biophys Res Commun 32:474–572

    Google Scholar 

  36. Chen XM, Guo GL, Sun L, Yang QS, Wang GQ, Qin GX (2016) Effects of Ala-Gln feeding strategies on growth, metabolism, and crowding stress resistance of juvenile Cyprinus carpio var. Jian Fish Shellfish Immunol 51:365–372

    Article  CAS  PubMed  Google Scholar 

  37. Iwama GK, Thomas PT, Forsyth RB, Vijayan MM (1998) Heat shock protein expression in fish. Rev Fish Biol Fish 8:35–56

    Article  Google Scholar 

  38. Lei AY, Zeng DG (2008) Effects of compound Chinese herbal on the expression of heat stress protein 70 gene in white shrimp (Litopenaeus vannamei). Guangxi Agric Sci 7:52–59

    Google Scholar 

  39. Dong Y, Dong S, Meng X (2008) Effects of thermal and osmotic stress on growth, osmoregulation and Hsp70 in sea cucumber (Apostichopus japonicus Selenka). Aquaculture 276:179–186

    Article  CAS  Google Scholar 

  40. Zhou C, Lin H, Huang Z, Wang J, Wang Y, Yu W (2015) Effects of dietary soybean isoflavones on non-specific immune responses and hepatic antioxidant abilities and mRNA expression of two heat shock proteins (HSPs) in juvenile golden pompano Trachinotus ovatus under pH stress. Fish Shellfish Immunol 47:1043–1050

    Article  CAS  PubMed  Google Scholar 

  41. Liu B, Ge X, Xie J, Xu P, He Y, Cui Y (2012) Effects of anthraquinone extract from Rheum officinale Bail on the physiological responses and HSP70 gene expression of Megalobrama amblycephala under Aeromonas hydrophila infection. Fish Shellfish Immunol 32:1–7

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

The research was supported by the earmarked fund for Modern Agro-industry Technology Research System (CARS-46) and National Natural Science Foundation of China (No. 31372540).

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Authors and Affiliations

  1. College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, Jilin, China

    Mu-Yang Li, Xin-Ming Zhu, Xiao-Tian Niu, Xiu-Mei Chen, Jia-Xin Tian, Yi-Di Kong, Dong-Ming Zhang, Lei Zhao & Gui-Qin Wang

  2. Ministry of Education Laboratory of Animal Production and Quality Security, Jilin Agricultural University, Changchun, 130118, Jilin, China

    Mu-Yang Li, Xin-Ming Zhu, Xiao-Tian Niu, Xiu-Mei Chen, Jia-Xin Tian, Yi-Di Kong, Dong-Ming Zhang, Lei Zhao & Gui-Qin Wang

  3. Jilin Provincial Key Laboratory of Animal Nutrition and Feed Science, Jilin Agricultural University, Changchun, 130118, Jilin, China

    Mu-Yang Li, Xin-Ming Zhu, Xiao-Tian Niu, Xiu-Mei Chen, Jia-Xin Tian, Yi-Di Kong, Dong-Ming Zhang, Lei Zhao & Gui-Qin Wang

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Li, MY., Zhu, XM., Niu, XT. et al. Effects of dietary Allium mongolicum Regel polysaccharide on growth, lipopolysaccharide-induced antioxidant responses and immune responses in Channa argus. Mol Biol Rep 46, 2221–2230 (2019). https://doi.org/10.1007/s11033-019-04677-y

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