Molecular Biology Reports

, Volume 46, Issue 2, pp 2059–2066 | Cite as

The anti-cancer effect of amygdalin on human cancer cell lines

  • Asghar Arshi
  • Sayed Mostafa HosseiniEmail author
  • Fataneh Saleh Khaje Hosseini
  • Zahra Yousefnejad Amiri
  • Fatemeh Sadat Hosseini
  • Mahsa Sheikholia Lavasani
  • Hossein Kerdarian
  • Maryam Safarpour Dehkordi
Original Article


Derived from rosaceous plant seed, amygdalin belongs to aromatic cyanogenic glycoside group, and its anticancer effects have been supported by mounting evidence. In this study, we objected to investigate amygdalin effect on two antiapoptotic genes (Survivin, XIAP) and two lncRNAs (GAS5, MALAT1) in human cancer cells (A549, MCF7, AGS). Employing RT-qPCR analysis, we compared the mRNA levels of the genes related to apoptosis in A549, MCF7, and AGS cancer cells between amygdalin-treated (24, 48 and 72 h) and un-treated groups. RNA was extracted from both cell groups and then cDNAs were synthesized. The changes in the gene expression levels were specified using ΔΔCt method. RT-qPCR analysis has revealed that the expression of Survivin, XIAP, GAS5 and MALAT1 in amygdala-treated cancer cells were significantly different, compared to the un-treated cells. However, these expressions were different depending on the treatment time. According to the results, amygdalin significantly inhibited the expression level of Survivin, and XIAP genes in treated via untreated group. Our findings suggest that amygdalin might have an anticancer effect due to the various gene expressions in A549, MCF7, and AGS human cancer cells, showing it’s potential as a natural therapeutic anticancer drug.


Amygdalin Antiapoptotic genes LncRNAs Cancer cell line 



Iran’s National Elites Foundation give this opportunity to graduate students to work on a scientific project instead of going to the military garrison. This manuscript is an outcome of military service project of first author (Mr. Asghar Arshi). Also, the authors would like to thank all the staff members of the Cellular and Molecular Research Center, Shahrekord University of Medical Sciences in Iran for their sincere support.

Author contributions

AA and SMH: Conceptualization; FH: Software; ZYA: Validation; FSH and ML: Formal analysis; SMH: Investigation; HK and MSD: Resources; AA and SMH: Writing-original draft preparation, Writing-review & editing; SMH: Supervision.

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants performed by any of the authors.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Human Genetic Research CenterBaqiyatallah University of Medical ScienceTehranIran
  2. 2.Department of Biology, Science and Research BranchIslamic Azad UniversityTehranIran
  3. 3.Students Research CommitteeBabol University of Medical ScienceBabolIran
  4. 4.Young Researchers and Elite Club, Shahrekord BranchIslamic Azad UniversityShahrekordIran

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