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The anticancer activity of visnagin, isolated from Ammi visnaga L., against the human malignant melanoma cell lines, HT 144

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Abstract

Melanoma is a cancer of melanocyte cells and has the highest global incidence. There is a need to develop new drugs for the treatment of this deadly cancer, which is resistant to currently used treatment modalities. We investigated the anticancer activity of visnagin, a natural furanochromone derivative, isolated from Ammi visnaga L., against malignant melanoma (HT 144) cell lines. The singlet oxygen production capacity of visnagin was determined by the RNO bleaching method while cytotoxic activity by the MTT assay. Further, HT 144 cells treated with visnagin were also exposed to visible light (λ ≥ 400 nm) for 25 min to examine the illumination cytotoxic activity. The apoptosis was measured by flow cytometry with annexin V/PI dual staining technique. The effect of TNF-α secretion on apoptosis was also investigated. In standard MTT assay, visnagin (100 µg/mL) exhibited 80.93% inhibitory activity against HT 144 cancer cell lines, while in illuminated MTT assay at same concentration it showed lesser inhibitory activity (63.19%). Visnagin was induced apoptosis due to the intracellular generation of reactive oxygen species (ROS) and showed an apoptotic effect against HT 144 cell lines by 25.88%. However, it has no effect on TNF-α secretion. Our study indicates that visnagin can inhibit the proliferation of malignant melanoma, apparently by inducing the intracellular oxidative stress.

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Acknowledgements

This study is a part of F.A.Ö’s Ph.D. thesis and was supported by the Research Fund of Istanbul University (Project Number: TP-19969). Prof. Dr. KerimanGünaydın would like to thank all staff of the Dr. Panjwani Center for Molecular Medicine and Drug Research (ICCBS), University of Karachi, Pakistan, for providing research facilities for her studies.

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Authors and Affiliations

  1. Department of Molecular Biology and Genetics, Faculty of Science, Istanbul University, 34134, Istanbul, Turkey

    Fatma Aydoğmuş-Öztürk & Keriman Günaydın

  2. Köyceğiz Vocational School, Muğla Sıtkı Koçman University, Köyceğiz, 48800, Muğla, Turkey

    Fatma Aydoğmuş-Öztürk

  3. Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Science, University of Karachi, Karachi, 75270, Pakistan

    Fatma Aydoğmuş-Öztürk, Humera Jahan & Muhammad Iqbal Choudhary

  4. Department of Chemistry, Faculty of Arts and Sciences, Mustafa Kemal University, 31060, Hatay, Turkey

    Neslihan Beyazit

  5. H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Science, University of Karachi, Karachi, 75270, Pakistan

    Muhammad Iqbal Choudhary

  6. Department of Biochemistry, Faculty of Science, King Abdulaziz University, 21412, Jeddah, Saudi Arabia

    Muhammad Iqbal Choudhary

Authors
  1. Fatma Aydoğmuş-Öztürk
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  2. Humera Jahan
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  4. Keriman Günaydın
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  5. Muhammad Iqbal Choudhary
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Correspondence to Keriman Günaydın.

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Aydoğmuş-Öztürk, F., Jahan, H., Beyazit, N. et al. The anticancer activity of visnagin, isolated from Ammi visnaga L., against the human malignant melanoma cell lines, HT 144. Mol Biol Rep 46, 1709–1714 (2019). https://doi.org/10.1007/s11033-019-04620-1

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  • DOI: https://doi.org/10.1007/s11033-019-04620-1

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