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Genome-wide analysis of the MATE gene family in potato

Molecular Biology Reports volume 46, pages 403–414 (2019)Cite this article

Abstract

The multidrug and toxic compound extrusion (MATE) protein family is a newly discovered family of secondary transporters that extrude metabolic waste and a variety of antibiotics out of the cell using an electrochemical gradient of H+ or Na+ across the membrane. The main function of MATE gene family is to participate in the process of plant detoxification and morphogenesis. The genome-wide analysis of the MATE genes in potato genome was conducted. At least 48 genes were initially identified and classified into six subfamilies. The chromosomal localization of MATE gene family showed that they could be distributed on 11 chromosomes except chromosome 9. The number of amino acids is 145–616, the molecular weight of proteins is 15.96–66.13 KD, the isoelectric point is 4.97–9.17, and they were located on the endoplasmic reticulum with having 4–13 transmembrane segments. They contain only two parts of the exons and UTR without introns. Some members of the first subfamily of potato MATE gene family are clustered with At2g04070 and they may be related to the transport of toxic compounds such as alkaloids and heavy metal. The function of the members of the second subfamily may be similar to that of At3g23560, which is related to tetramethylammonium transport. Some members of the third subfamily are clustered with At3g59030 and they may be involved in the transport of flavonoids. The fifth subfamily may be related to the transport of iron ions. The function of the sixth subfamily may be similar to that of At4g39030, which is related to salicylic acid transport. There are three kinds of conserved motifs in potato MATE genes, including the motif 1, motif 2, and motif 3. Each motif has 50 amino acids. The number of each motif is different in the gene sequence, of which 45 MATE genes contain at least a motif, but there is no motif in ST0015301, ST0045283, and ST0082336. These results provide a reference for further research on the function of potato MATE genes.

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Acknowledgements

This study was supported by grants from the National Natural Science Foundation of China (Grant Nos. 31660352, 31660350) and the Science & Technology Development Fund of Guangxi Academy of Agricultural Sciences (Grant Nos. Guinongke2017JZ11 and Guinongke2017JZ21).

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Affiliations

  1. College of Agronomy, Guangxi University, Nanning, 530004, People’s Republic of China

    Yinqiu Li & Long-Fei He

  2. Cash Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, 530007, People’s Republic of China

    Huyi He

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  1. Yinqiu Li
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Li, Y., He, H. & He, LF. Genome-wide analysis of the MATE gene family in potato. Mol Biol Rep 46, 403–414 (2019). https://doi.org/10.1007/s11033-018-4487-y

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  • DOI: https://doi.org/10.1007/s11033-018-4487-y

Keywords

  • Potato
  • MATE gene family
  • Protein characteristics
  • Phylogenetic evolution
  • Domain
  • Function