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Bioguided isolation, characterization and media optimization for production of Lysolipins by actinomycete as antimicrobial compound against Xanthomonas citri subsp. citri

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Abstract

Citrus Canker disease is one of the most important disease in citrus production worldwide caused by gram-negative bacterial pathogen Xanthomonas citri subsp. citri, leading to great economic losses. Currently, a spray of copper-based bactericides is the primary measure for citrus canker management. However, these measures can lead to the contamination of soil by metal contamination, but also the development of copper-resistant Xanthomonas populations. Considering the need to discovery new alternatives to control the citrus canker disease, actinomycetes isolated from the Brazilian Caatinga biome and their crude extracts were tested against different strains of Xanthomonas citri subsp. citri. Streptomyces sp. Caat 1-54 crude extract showed the highest antibiotic activity against Xcc. The crude extract dereplication was performed by LC–MS/MS. Through bioassay-guided fractionation strategy, the antimicrobial activity was assigned to Lysolipins, showing a MIC around 0.4–0.8 µg/mL. Growth media optimization using statistical experimental design increased the Lysolipins production in three-fold production. The preventive and curative effects of the optimized crude extract obtained by experimental design of Caat-1-54 against citrus canker were evaluated in potted ‘Pera’ sweet orange nursery trees. Caat 1-54 extract was effective in preventing new infections by Xcc on leaves but was not able to reduce Xcc population in pre-established citrus canker lesions. Streptomyces sp. Caat 1-54 extract is a promising, environmentally-friendly source of antimicrobial compound to protect citrus trees against citrus canker.

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Abbreviations

CID:

Collision-induced dissociation

RSM:

Response surface methodology

MIC:

Minimum inhibitory concentration

DMSO:

Dimethyl sulfoxide

MTT:

Thiazolyl blue tetrazolium bromide

TLC:

Thin-layer chromatography

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Funding

This work was supported by CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) and FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo) - process (2016-22023-0) with financial support and fellowships.

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Author notes

  1. Júlia Pereira Rodrigues and Ana Paula Ferranti Peti have contributed equally to this work.

Authors and Affiliations

  1. Department of Chemistry, Faculty of Philosophy, Science and Letters, University of São Paulo, Bandeirantes Avenue, 3900, Monte Alegre, Ribeirão Preto, São Paulo, 12040-901, Brazil

    Júlia Pereira Rodrigues, Ana Paula Ferranti Peti, Fernanda Salés Figueiró, Izadora de Souza Rocha, Vinicius Ricardo Acquaro Junior & Luiz Alberto Beraldo Moraes

  2. FUNDECITRUS, Fundo de Defesa da Citricultura, Araraquara, Brazil

    Tamires Garcia Silva & Franklin Behlau

  3. EMBRAPA, Empresa Brasileira de Pesquisa Agropecuária, Jaguariúna, Brazil

    Itamar Soares de Melo

Authors
  1. Júlia Pereira Rodrigues
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  2. Ana Paula Ferranti Peti
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  3. Fernanda Salés Figueiró
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Correspondence to Luiz Alberto Beraldo Moraes.

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Rodrigues, J.P., Peti, A.P.F., Figueiró, F.S. et al. Bioguided isolation, characterization and media optimization for production of Lysolipins by actinomycete as antimicrobial compound against Xanthomonas citri subsp. citri. Mol Biol Rep 45, 2455–2467 (2018). https://doi.org/10.1007/s11033-018-4411-5

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