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Microbe-mediate transformation of echinocystic acid by whole cells of filamentous fungus Cunninghamella blakesleana CGMCC 3.910

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Abstract

Structural modification of echinocystic acid (EA), a pentacyclic triterpenoid with wide spread biological activities was investigated by microbial transformation. Microbe-mediate transformation of EA was carried out by filamentous fungus Cunninghamella blakesleana CGMCC 3.910. Four metabolites 3β, 7β, 16α-trihydroxy-olean-12-en-28-oic acid (EA-2); 3β, 7β, 16β,19β-tetrahydroxy-olean-12-en-28-oic acid (EA-3); 3β, 7β, 16α, 21β-tetrahydroxy-olean-12-en-28-oic acid (EA-4); 3β, 7β, 16α-trihydroxy-olean-11, 13(18)-dien-28-oic acid (EA-5) were produced. Structures of transformed products were elucidated by 1D and 2D NMR and HR-MS data. EA-3 and EA-4 were new compounds.

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Acknowledgements

This research was financially supported by The National Natural Sciences Foundation of China (No. 21462057, 21172267), Guizhou Science and Technology Department (QKHLH-2014-7555).

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Author notes

  1. Shaobin Fu and Xu Feng contributed equally to this work.

Authors and Affiliations

  1. Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100193, China

    Shaobin Fu, Xu Feng & Di-an Sun

  2. Department of Pharmacy, Zunyi Medical University, Zunyi, 563000, China

    Shaobin Fu

  3. Navy Medical Research Institute Second Military Medical University, Shanghai, 200433, China

    Xu Feng

Authors
  1. Shaobin Fu
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  2. Xu Feng
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  3. Di-an Sun
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Correspondence to Shaobin Fu or Di-an Sun.

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Fu, S., Feng, X. & Sun, Da. Microbe-mediate transformation of echinocystic acid by whole cells of filamentous fungus Cunninghamella blakesleana CGMCC 3.910. Mol Biol Rep 45, 2795–2800 (2018). https://doi.org/10.1007/s11033-018-4357-7

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  • DOI: https://doi.org/10.1007/s11033-018-4357-7

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