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Induction of apoptotic effects of anti-proliferative zeolite X from coal fly ash on cervical cancer (HeLa) cell lines

Molecular Biology Reports volume 45, pages 1077–1087 (2018)Cite this article

Abstract

The synthesised zeolite X from coal fly ash showed significant cytotoxic activity in contradiction of HeLa cells (cervical cancer) in a concentration-dependent way at concentrations ranges from 200 µg to 0.781 µg/ml as shown by MTT assay and failed to cause cytotoxic effect in normal cells (Gh239). Cell cycle analysis exposed that zeolite X (10 and 15 µg/ml) endorses cell growth inhibition by inducing G2/M phase arrest in HeLa cells as observed using flow cytometry. The confocal microscopic results depicted increased early apoptotic related changes in HeLa cell lines induced by zeolite X at a dosage of 10, 15 and 20 µg/ml. Zeolite X at a dosage of 10, 15 and 20 µg/ml in HeLa cells showed fragmentation of DNA by ladder pattern thereby indicates that cell death is related with apoptosis. By the increase of Bax/Bcl-2 ratio, zeolite X leads to the caspase-3 and caspase-9 activation and allow the cells to enter apoptosis. These collective results evidently showed that the influence of mitochondria-mediated signalling pathway in zeolite X induced apoptosis and intensely delivered investigational suggestion for the use of zeolite X as a significant curative agent in the preclusion and therapy of human cervical carcinoma.

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Affiliations

  1. Department of Chemical Engineering, Anna University, A.C. Tech Campus, Chennai, Tamil Nadu, 600 025, India

    S. Subhapriya & P. Gomathipriya

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  1. S. Subhapriya
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  2. P. Gomathipriya
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Correspondence to P. Gomathipriya.

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Subhapriya, S., Gomathipriya, P. Induction of apoptotic effects of anti-proliferative zeolite X from coal fly ash on cervical cancer (HeLa) cell lines. Mol Biol Rep 45, 1077–1087 (2018). https://doi.org/10.1007/s11033-018-4259-8

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  • DOI: https://doi.org/10.1007/s11033-018-4259-8

Keywords

  • Apoptosis
  • Cell cycle analysis
  • DNA fragmentation
  • Gh239 cells
  • HeLa cells
  • Zeolite X