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The association between cardiovascular disease gene mutations and recurrent pregnancy loss in the Lebanese population

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Abstract

Recurrent pregnancy loss (RPL) is a problem affecting up to 5% of women of childbearing age due to many factors. Studies have shown that RPL and cardiovascular disease (CVD) may have shared risk factors. We compared the prevalence of 12 cardiovascular disease related gene mutations in patients with a history of RPL to normal controls in a major tertiary care center in Lebanon. The CVD StripAssay (ViennaLab, Austria) was used to analyze the CVD genes on 70 women with RPL history as part of the initial routine workup for recurrent miscarriage at the American University of Beirut Medical Center. The obtained results were compared with data of controls from the Lebanese population using Fisher’s exact test and Chi square analysis. Two genes of the CVD panel demonstrated a strong relationship with RPL, including, MTHFR (C677T homozygosity, A1298C homozygosity, and compound heterozygosity for C677T and A1298C) and Factor II (heterozygosity for G20210A). Moreover, a protective role of positive APO-E3 isoform was observed. This study is the first in the Lebanese population in associating RPL with a large panel of CVD related genes.

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Author notes

  1. Hanadi El Achi and Johnny Awwad have contributed equally to this research work and manuscript and should both be considered as first authors.

Authors and Affiliations

  1. Department of Pathology and Laboratory Medicine, American University of Beirut Medical Center, Cairo Street, Beirut, Lebanon

    Hanadi El Achi, Sarah Abou Daya, Sahar Halabi, Sandra Damianos & Rami Mahfouz

  2. Department of Obstetrics and Gynecology, American University of Beirut Medical Center, Beirut, Lebanon

    Johnny Awwad

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  1. Hanadi El Achi
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Correspondence to Rami Mahfouz.

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Research involving human participants and all procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Informed consent was waived by the Intuitional Review Board of the American University of Beirut Medical Center.

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This article does not contain any studies on humans or animals. It was a retrospective review of data.

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El Achi, H., Awwad, J., Abou Daya, S. et al. The association between cardiovascular disease gene mutations and recurrent pregnancy loss in the Lebanese population. Mol Biol Rep 45, 911–916 (2018). https://doi.org/10.1007/s11033-018-4237-1

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  • DOI: https://doi.org/10.1007/s11033-018-4237-1

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