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Molecular Biology Reports

, Volume 45, Issue 4, pp 633–638 | Cite as

Effect of master mixes on the measurement of telomere length by qPCR

  • Karen M. Jiménez
  • Diego A. Forero
Short Communication

Abstract

Alterations in telomere length (TL) have been associated with several diseases and a method based on qPCR, the Monochrome Multiplex Real-Time Quantitative PCR (MMQPCR) technique, has been used extensively for the analysis of TL. Some previous studies have been found that certain methodological conditions can affect the measurement of TL. The aim of the study was to evaluate the performance of eight different commercially available SYBR Green and High-Resolution Melting (HRM) mixes on the measurement of TL by the MMQPCR method. Four SYBR Green and four HRM mixes were tested and the measurement of TL was expressed by the T/S ratio. It was found that the type of master mix used in MMQPCR influences the measurement of TL, affecting aspects such as the specificity and consistency of the results. Our results are the first description of the effects of different master mixes on TL analysis by MMQPCR and highlight the importance of the future methodological improvement of this broadly used technique.

Keywords

Epigenetics Molecular genetics Molecular methods qPCR Telomere length 

Notes

Acknowledgements

This study was supported by research grants from Colciencias (Grant No. 823-2015) and VCTI (Grant No. 2016220).

Compliance with ethical standards

Conflict of interest

The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in this study.

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Laboratory of NeuroPsychiatric Genetics, Biomedical Sciences Research Group, School of MedicineUniversidad Antonio NariñoBogotáColombia

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