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Development of microsatellite markers for Manilkara maxima T.D. Penn. (Sapotaceae) and their use in conservation genetics

Molecular Biology Reports volume 43, pages 451–455 (2016)Cite this article

Abstract

Manilkara maxima is an endemic tree species of the Atlantic Forest in southern Bahia, Brazil. It is considered important for forest conservation due to its mutualistic interactions with endemic and endangered animals. Our aim was to develop microsatellite markers to estimate genetic diversity in order to provide information for effectiveness of future conservation programs. We used next generation sequencing technology to develop the first specific microsatellite markers for M. maxima. Seventeen new microsatellite loci were applied in 72 individuals sampled in three natural populations. On average, the number of alleles per loci was 8.8. The expected heterozygosity varied between 0.72 and 0.77, indicating that the developed set of molecular markers is useful for genetic diversity studies. Additionally, the estimated value for the combined probability of exclusion (Q) was greater than 0.999, which indicates the powerful of these molecular tools for paternity and kinship analysis. Our results demonstrate that the set of microsatellites developed in this work is a powerful tool for population genetics, molecular ecology and conservation biology purposes.

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Acknowledgments

We thank Horlei Ribeiro, Rodrigo Bezerra, Daniela Borges and Jiomário Souza, for field and laboratory support. This work was funded by CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico—SISBIOTA# 563216/2010-7)/FAPESB (Fundação de Amparo à Pesquisa do Estado da Bahia) (PRONEX#PNX0014/2009). We also thank FAPESB for scholarship to JASJ and CNPq for research fellowship for FAG.

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Authors and Affiliations

  1. Pós-graduação em Genética e Biologia Molecular, Universidade Estadual de Santa Cruz (UESC), Rodovia Ilhéus-Itabuna km 16 s/n, Salobrinho, Ilhéus, Bahia, 45662-900, Brazil

    José Audenor Silva-Junior, Daniele de Souza França & Fernanda Amato Gaiotto

  2. Departamento de Ciências Biológicas, Laboratório de Marcadores Moleculares, Centro de Biotecnologia e Genética, Universidade Estadual de Santa Cruz (UESC), Rodovia Ilhéus-Itabuna km 16 s/n, Salobrinho, Ilhéus, Bahia, 45662-900, Brazil

    Fernanda Amato Gaiotto

  3. Pós-graduação em Ecologia e Conservação da Biodiversidade, Universidade Estadual de Santa Cruz (UESC), Rodovia Ilhéus-Itabuna km 16 s/n, Salobrinho, Ilhéus, Bahia, 45662-900, Brazil

    Ramiris César Souza Moraes

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  1. José Audenor Silva-Junior
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  2. Daniele de Souza França
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  3. Ramiris César Souza Moraes
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  4. Fernanda Amato Gaiotto
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Correspondence to Fernanda Amato Gaiotto.

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Silva-Junior, J.A., de Souza França, D., Moraes, R.C.S. et al. Development of microsatellite markers for Manilkara maxima T.D. Penn. (Sapotaceae) and their use in conservation genetics. Mol Biol Rep 43, 451–455 (2016). https://doi.org/10.1007/s11033-016-3981-3

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  • DOI: https://doi.org/10.1007/s11033-016-3981-3

Keywords

  • Conservation
  • Tropical forest
  • SSR
  • Population genetics
  • Molecular markers
  • NGS