Abstract
In paternity cases where individuals are close relatives, it may be necessary to evaluate mother’s DNA profile (trio test) and to increase the number of polymorphic STR loci that are analyzed. In our case, two alleged fathers who are brothers and the child (duo case) were analyzed based on 20 STR loci; however, no exclusions could be achieved. Then trio test (with mother) was performed using the Identifiler Plus kit (Applied Biosystems) and no exclusions could be achieved again. Analysis performed with the ESS Plex Plus kit (Qiagen), the paternity of one of the two alleged fathers was rejected only on 2 STR loci. We made the calculations of power of exclusion values to interpret our results more properly. The probability of exclusion (PE) is calculated as 0.9776546 in 15 loci of Identifiler Plus kit without mother. The PE is calculated as 0.9942803, if 5 additional loci from ESS Plex Plus kit are typed. The PE becomes 0.9961048 for the Identifiler Plus kit in trio analysis. If both Identifiler Plus and ESS Plex Plus kits are used for testing, the PE is calculated as 0.999431654, which indicates that the combined kits are highly discriminating.
This is a preview of subscription content, log in via an institution to check access.
Explore related subjects
Discover the latest articles and news from researchers in related subjects, suggested using machine learning.References
Gonzalez-Andrade F, Sanchez D, Penacino G, Martinez Jarreta B (2009) Two fathers for the same child: a deficient paternity case of false inclusion with autosomic STRs. Forensic Sci Int Genet 3:138–140
Poetsch M, Ludcke C, Repenning A, Fisher L, Malyusz V, Simeoni E, Lignitz E, Oehmichen M, von Wurmb-Schwark N (2006) The problem of single parent/child paternity analysis—practical results involving 336 children and 348 unrelated men. Forensic Sci Int 159:98–103
Canturk KM, Emre R, Muslumanoglu O, Huner AS, Uygun H, Bingöl A, Aksoy HY, Kınoglu K (2014) Brother-sister incest paternity case with the same tri-allelic pattern of parents at D3S1358 locus: a tetra-allelic baby? Int J Legal Med. doi:10.1007/s00414-014-0984-2
Von Wurmb-Schwark N, Ma´ lyusz V, Simeoni E, Lignitz E, M Poetsch (2006) Possible pitfalls in motherless paternity analysis with related putative fathers. Forensic Sci Int 159:92–97
Borsting C, Morling N (2011) Mutations and/or close relatives? Six case work examples where 49 autosomal SNPs were used as supplementary markers. Forensic Sci Int Genet 5:236–241
Edelmann J, Lessig R, Klintschar M, Szibor R (2004) Advantages of X-chromosomal microsatellites in deficiency paternity testing: presentation of cases. Int Congr Ser 1261:257–259
Canturk KM, Emre R, Kınoglu K, Başpınar B, Sahin F, Ozen M (2014) Current status of the use of single-nucleotide polymorphisms in forensic practices. Genet Test Mol Biomark 18(7):45–60
Fung WK, Chung YK, Wong DM (2002) Power of exclusion revisited: probability of excluding relatives of the true father from paternity. Int J Leg Med 116:64–67
Garber RA, Morris JW (1983) General equations for the average power of exclusion for genetic systems of n co-dominant alleles in one-parent and in no-parent cases of disputed parentage. Inclusion Probabilities in Parentage Testing. American Association of Blood Banks, Arlington, pp 277–280
Fung WK, Wong DM, Chung YK (2003) How well do serial tandem repeat loci perform in excluding paternity in relatives of the biological father among immigration cases? Transfusion 43:982–983
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Dogan, M., Kara, U., Emre, R. et al. Two brothers’ alleged paternity for a child: who is the father?. Mol Biol Rep 42, 1025–1027 (2015). https://doi.org/10.1007/s11033-014-3839-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11033-014-3839-5