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Phylogenetic analysis of the Listeria monocytogenes based on sequencing of 16S rRNA and hlyA genes

Molecular Biology Reports volume 41, pages 8219–8229 (2014)Cite this article

Abstract

The discrimination between Listeria monocytogenes and Listeria species has been detected. The 16S rRNA and hlyA were PCR amplified with set of oligonucleotide primers with flank 1,500 and 456 bp fragments, respectively. Based on the differences in 16S rRNA and hlyA genes, a total 80 isolates from different environmental, food and clinical samples confirmed it to be L. monocytogenes. The 16S rRNA sequence similarity suggested that the isolates were similar to the previously reported ones from different habitats by others. The phylogenetic interrelationships of the genus Listeria were investigated by sequencing of 16S rRNA and hlyA gene. The 16S rRNA sequence indicated that genus Listeria is comprised of following closely related but distinct lines of descent, one is the L. monocytogenes species group (including L. innocua, L. ivanovii, L. seeligeri and L. welshimeri) and other, the species L. grayi, L. rocourtiae and L. fleischmannii. The phylogenetic tree based on hlyA gene sequence clearly differentiates between the L. monocytogenes, L. ivanovii and L. seeligeri. In the present study, we identified 80 isolates of L. monocytogenes originating from different clinical, food and environmental samples based on 16S rRNA and hlyA gene sequence similarity.

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Acknowledgments

This study was supported by Indian Council of Medical Research (ICMR), Government of India, New Delhi through the research project No. 5/3/3/10/2007-ECD-I.

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  1. Environmental Microbiology Lab, Department of Botany, Banaras Hindu University, Varanasi, 221005, India

    Dharmendra Kumar Soni & Suresh Kumar Dubey

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  1. Dharmendra Kumar Soni
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  2. Suresh Kumar Dubey
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Correspondence to Suresh Kumar Dubey.

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Soni, D.K., Dubey, S.K. Phylogenetic analysis of the Listeria monocytogenes based on sequencing of 16S rRNA and hlyA genes. Mol Biol Rep 41, 8219–8229 (2014). https://doi.org/10.1007/s11033-014-3724-2

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  • DOI: https://doi.org/10.1007/s11033-014-3724-2

Keywords

  • L. monocytogenes
  • 16S rRNA
  • hlyA
  • Sequencing
  • Phylogenetic analysis