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Transcriptome profiling of biliary atresia from new born infants by deep sequencing

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Abstract

Biliary atresia is the major kind of liver disease that mainly affects the new born infants. The pathological and biological mechanism of biliary atresia is still unclear to date. In this work, we attempt to identify biliary atresia relevant genes and to get the knowledge of the underlying genetic basis. We collected liver samples from new born infants with biliary atresia and congenital choledochocyst, and the RNA-seq technology was used to performed a transcriptome profiling in order to comprehensively study their expression signatures. We identified 877 differentially expressed genes between samples from biliary atresia and congenital choledochocyst patients in total. Several biological pathways related to the immunity and inflammation response were found to involve in the development of biliary atresia. Our results may helps to better investigate the molecular mechanisms of this disease.

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Acknowledgments

This work is supported by Young talent project of Shanghai Board of health and the funding No. is 2012Y131.

Conflict of interest

The authors declare that there is no conflict of interest.

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Authors and Affiliations

  1. Ren Ji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China

    Jie Xiao, Su-yun Xia, Yun Xia, Qiang Xia & Xiang-rui Wang

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  1. Jie Xiao
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  2. Su-yun Xia
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  3. Yun Xia
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Correspondence to Xiang-rui Wang.

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Xiao, J., Xia, Sy., Xia, Y. et al. Transcriptome profiling of biliary atresia from new born infants by deep sequencing. Mol Biol Rep 41, 8063–8069 (2014). https://doi.org/10.1007/s11033-014-3704-6

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  • DOI: https://doi.org/10.1007/s11033-014-3704-6

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