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Protective effect of myokine IL-15 against H2O2-mediated oxidative stress in skeletal muscle cells

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Abstract

The production of reactive oxygen species (ROS) during oxidative stress may cause cellular injury. Interleukin-15 (IL-15) is one of the skeletal muscle secreted myokines, and there is no information that reported its anti-oxidative capability in skeletal muscle. The aim of this study therefore is to investigate the protective effects of myokine IL-15 against H2O2-mediated oxidative stress in C2C12 myoblasts. The results showed that IL-15 pre-incubation reduced the intracellular creatine kinase and lactate dehydrogenase activities, decreased the ROS overload, and protect the mitochondrial network via up-regulated mRNA expression levels of IL-15 and uncoupling protein 3. It also down-regulated the levels of IL-6 and p21 of the myoblasts compared to the cells treated only with H2O2. Meanwhile, apurinic/aprimidinic endonuclease 1 expression and the Akt signaling pathway were stimulated. These effects could contribute to the resumption of cell viability and act as protective mechanism. In conclusion, myokine IL-15 could be a novel endogenous regulator to control intracellular ROS production and attenuate oxidative stress in skeletal muscle cells.

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Abbreviations

APE1:

Apurinic/aprimidinic endonuclease 1

CK:

Creatin kinase

DMEM:

Dulbecco’s modified Eagle’s medium

ERK1/2:

Extracellular-signal regulated kinase-1/2

LDH:

Lactate dehydrogenase

MyoD:

Myogenic differentiation antigen

PBS:

Phosphate buffered saline

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

UCP3:

Uncoupling protein 3

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Acknowledgments

This study was jointly supported by National Basic Research Program of China (2013CB127305, 2012CB124704), National Nature Science Foundation of China (31372325, 31110103909), and the Project of Institute of Subtropical Agriculture, the Chinese Academy of Sciences (ISACX-LYQY-QN-1104). The authors’ contributions were as follows: F. N. Li and Y. L. Yin were in charge of the whole trial. F. N. Li wrote the manuscript while A.O Oso assisted in technical editing and correction of the paper. Y. H. Li, Y. L. Tan and B. B. Lin assisted with the cell culture. X. F. Kong assisted with the biochemical analyses.

Conflict of interest

The authors have declared that no competing interests exist.

Author information

Authors and Affiliations

  1. Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, Hunan, China

    Fengna Li, Yinghui Li, Yulong Tang, Binbin Lin, Xiangfeng Kong & Yulong Yin

  2. University of Chinese Academy of Sciences, Beijing, 100039, China

    Yinghui Li

  3. College of Animal Sciences, Hunan Agricultural University, Changsha, 410128, Hunan, China

    Binbin Lin

  4. Animal Nutrition Department, College of Animal Science and Livestock Production, Federal University of Agriculture Abeokuta, P.M.B. 2240, Abeokuta, Ogun, Nigeria

    Oso Abimbola Oladele

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  1. Fengna Li
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Correspondence to Yulong Yin.

Additional information

Fengna Li and Yinghui Li contributed equally to the work.

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Li, F., Li, Y., Tang, Y. et al. Protective effect of myokine IL-15 against H2O2-mediated oxidative stress in skeletal muscle cells. Mol Biol Rep 41, 7715–7722 (2014). https://doi.org/10.1007/s11033-014-3665-9

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  • DOI: https://doi.org/10.1007/s11033-014-3665-9

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