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Molecular characterization of PXR and two sulfotransferases and hepatic transcripts of PXR, two sulfotransferases and CYP3A responsive to bisphenol A in rare minnow Gobiocypris rarus

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Abstract

Bisphenol A (BPA), a wide distributed endocrine-disrupting chemical, has attracted many attentions. To explore the effect of BPA on hepatic metabolic pathways in Gobiocypris rarus, full-length cDNAs of pregnane X receptor (PXR) and two sulfotransferases (SULT1 ST4 and SULT1 ST6) were firstly isolated and characterized. We detected tissues distribution of PXR, CYP3A, SULT1 ST4 and SULT1 ST6 in adult G. rarus. Then we investigated hepatic transcript profiles of these four genes in adult G. rarus exposed to BPA at concentrations of 5, 15, and 50 µg/L for 14 and 35 days. It demonstrates that these four genes are all highly expressed in liver of both male and female adult G. rarus. In response to BPA, sexual dimorphism of expression patterns for PXR, CYP3A, and SULT1 ST6 shows in G. rarus, which includes increase of mRNA levels in females and decrease of mRNA levels in males in both exposure durations of 14 and 35 days. SULT1 ST6 mRNA demonstrates high responsiveness to BPA in both genders and we recommended SULT1 ST6 as a candidate biomarker for BPA exposure.

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Acknowledgments

This study was supported by grants from the Natural Science Foundation of Shaanxi Province (2011JM3009) and the National Natural Science Foundation of China (31270547).

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  1. Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, 22 Xinong Road, Yangling, 712100, Shaanxi, China

    Jiancao Gao, Yingying Zhang, Yanping Yang, Cong Yuan, Fang Qin, Shaozhen Liu, Yao Zheng & Zaizhao Wang

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  1. Jiancao Gao
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Correspondence to Zaizhao Wang.

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11033_2014_3598_MOESM1_ESM.tif

Fig. 1 Phylogenetic tree of vertebrates PXR based on amino acid sequences. The phylogenic tree was constructed using MEGA 5.0 program with maximum likelihood algorithms method and bootstrap resampling (1,000 replications). Numbers in the branches represent the bootstrap values (%) from 100 replicates. GenBank accession numbers of the PXR amino acid sequences included in the tree are: ADL27416.1 (Ctenopharyngodon idella), CAQ14197.1 (Danio rerio), ABR21208.1 (Fundulus heteroclitus), AAH17304.2 (Homo sapiens), NP_001091874.1 (Mus musculus), NP_001118144.1 (Oncorhynchus mykiss), BAM36700.1 (Oreochromis niloticus), ABV29341.1 (Pimephales promelas), ABG01901.1 (Sus scrofa) and NP_001083606.1 (Xenopus laevis) (TIFF 285 kb)

11033_2014_3598_MOESM2_ESM.tif

Fig. 2 Phylogenetic tree of vertebrates SULT based on amino acid sequences. The phylogenic tree was constructed using MEGA 5.0 program with maximum likelihood algorithms method and bootstrap resampling (1,000 replications). Numbers in the branches represent the bootstrap values (%) from 100 replicates. GenBank accession numbers of the SULT amino acid sequences included in the tree are: CAQ15389.1 (Danio rerio SULT1 ST1), AAH47850.1 (Danio rerio SULT1 ST2), CAQ15393.1 (Danio rerio SULT1 ST3), AAH66584.1 (Danio rerio SULT1 ST4), NP_001186832.1 (Danio rerio SULT1 ST5), AAH75996.1 (Danio rerio SULT1 ST6), NP_001132953.1 (Danio rerio SULT1 ST7), NP_001132954.1 (Danio rerio SULT1 ST8), AFC93291.1 (Danio rerio SULT1 ST9), NP_944596.2 (Danio rerio SULT2 ST1), NP_001071637.1 (Danio rerio SULT2 ST2), NP_001071636.2 (Danio rerio SULT2 ST3), XP_687148.1 (Danio rerio SULT3 ST1), NP_001073416.1 (Danio rerio SULT3 ST2), NP_001076345.1 (Danio rerio SULT3 ST3), AFC93292.1 (Danio rerio SULT3 ST4), AAH00923.1 (Homo sapiens SULT1A1), NP_001045.1 (Homo sapiens SULT1A2), AAB31317.1 (Homo sapiens SULT1A3), NP_598431.1 (Mus musculus SULT1A1), BAA82321.1 (Mus musculus SULT1A4). DR, Danio rerio; GR,Gobiocypris rarus; H, Homo sapiens; M, Mus musculus (TIFF 5619 kb)

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Gao, J., Zhang, Y., Yang, Y. et al. Molecular characterization of PXR and two sulfotransferases and hepatic transcripts of PXR, two sulfotransferases and CYP3A responsive to bisphenol A in rare minnow Gobiocypris rarus . Mol Biol Rep 41, 7153–7165 (2014). https://doi.org/10.1007/s11033-014-3598-3

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