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Purification and mass spectrometry based characterization of a pediocin produced by Pediococcus acidilactici 13

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Abstract

Bacteriocins are antimicrobial peptides produced by several bacterial species. Among the bacteriocins pediocin-like bacteriocins have a significant inhibitory activity on the foodborne pathogens especially on Listeria monocytogenes. This study aims to select a simple and usable purification method to purify/concentrate the antimicrobial peptide and characterization of the bacteriocin produced by Pediococcus acidilactici 13 by using proteomic approaches which is a recent omic technology. For purification dialysis, ultrafiltration method was used, and as a result of this study the bacteriocin activity reached 819,200 AU/mL from 102,400 AU/mL initially. Two dimensional gel electrophoresis and then matrix-assisted laser desorption ionization/time of flight mass spectrometry (MALDI-TOF MS) analysis were carried out to identify the current bacteriocin and related proteins. Obtained data revealed similarity to pediocin PA-1 transport/processing ATP-binding protein PedD (accession number: P36497), pediocin operon PedC (accession number: Q68GC4) and bacteriocin pediocin PA-1 (accession number: P29430) from UniProtKB/Swiss-Prot databank, thus the bacteriocin produced by P. acidilactici 13 is considered similar to pediocin PA-1.

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Acknowledgments

Authors thank to Biotechnology Institution at the University of Ankara for enabling them to carry out proteomic studies in Central Laboratory. This study is based on a part of the Ph.D thesis of Evrim GUNES ALTUNTAS, submitted to Science Institution at the University of Ankara.

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Authors and Affiliations

  1. Central Laboratory, Biotechnology Institute, Ankara University, 06110, Tandoğan, Ankara, Turkey

    Evrim Güneş Altuntaş, Selen Peker, Beycan Ayhan & Duygu Özel Demiralp

  2. Food Engineering Department, Engineering Faculty, Ankara University, Ankara, Turkey

    Kamuran Ayhan

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  1. Evrim Güneş Altuntaş
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  2. Kamuran Ayhan
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  3. Selen Peker
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  4. Beycan Ayhan
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Correspondence to Evrim Güneş Altuntaş.

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Altuntaş, E.G., Ayhan, K., Peker, S. et al. Purification and mass spectrometry based characterization of a pediocin produced by Pediococcus acidilactici 13. Mol Biol Rep 41, 6879–6885 (2014). https://doi.org/10.1007/s11033-014-3573-z

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  • DOI: https://doi.org/10.1007/s11033-014-3573-z

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