Abstract
A multiplex primer-extension reaction (PER) assay, was specifically designed for the identification of ten Yersinia species. The assay, directed towards the tufA (elongation factor Tu) gene, was tested on a total of 42 samples representing Yersinia species and non-Yersinia species. The primers used in the preliminary PCR, designed in highly conserved regions upstream and downstream of the diagnosis sites, successfully amplified a 587 bp fragment. The diagnosis sites were simultaneously interrogated using a multiplex PER and the results were confirmed by fragment sequencing. The proposed test provides an appropriate tool to monitor the presence of Yersinia spp. in food samples and to evaluate the potential hazard for consumers.
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This research has been financially supported by a grant of University of Turin (ex 60 %).
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None of the authors of this paper has a financial or personal relationship with other people or organisations that could inappropriately influence or bias the content of the paper.
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Dalmasso, A., Civera, T., Filipello, V. et al. Multiplex primer-extension assay for identification of Yersinia species. Mol Biol Rep 41, 6329–6333 (2014). https://doi.org/10.1007/s11033-014-3555-1
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DOI: https://doi.org/10.1007/s11033-014-3555-1