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Analysis of S-locus and expression of S-alleles of self-compatible rapid-cycling Brassica oleracea ‘TO1000DH3’

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Abstract

Brassica oleracea is a strictly self-incompatible (SI) plant, but rapid-cycling B. oleracea ‘TO1000DH3’ is self-compatible (SC). Self-incompatibility in Brassicaceae is controlled by multiple alleles of the S-locus. Three S-locus genes, S-locus glycoprotein (SLG), S-locus receptor kinase (SRK) and S-locus protein 11 or S-locus cysteine-rich (SP11/SCR), have been reported to date, all of which are classified into class I and II. In this study, we investigated the molecular mechanism behind alterations of SI to SC in rapid-cycling B. olerace ‘TO1000DH3’. Class I SRK were identified by genomic DNA PCR and PCR-RFLP analysis using SRK specific markers and found to be homozygous. Cloning and sequencing of class I SRK revealed a normal kinase domain without any S-domain/transmembrane domain. Moreover, S-locus sequencing analysis revealed only an SLG sequence, but no SP11/SCR. Expression analysis showed no SRK expression in the stigma, although other genes involved in the SI recognition reaction (SLG, MLPK, ARC1, THL) were found to have normal expression in the stigma. Taken together, the above results suggest that structural aberrations such as deletion of the SI recognition genes may be responsible for the breakdown of SI in rapid-cycling B. oleracea ‘TO1000DH3’.

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Acknowledgments

This work was supported by the Cabbage Genomics assisted breeding supporting center research program funded by Ministry for Food, Agriculture, Forestry and Fisheries of the Korean Government.

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Authors and Affiliations

  1. Department of Horticulture, Sunchon National University, 413 Jungangno, Suncheon, Jeonnam, 540-742, Republic of Korea

    Jung Hee-Jeong, Ahmed Nasar Uddin, Park Jong-In, Thamilarasan Senthil Kumar & Nou Ill-Sup

  2. Plant Systems Engineering Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 305-806, Republic of Korea

    Kim Hye-Ran

  3. Department of Crop Science, Chungbuk National University, Cheongju, 361-763, Republic of Korea

    Cho Yong-Gu

Authors
  1. Jung Hee-Jeong
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  2. Ahmed Nasar Uddin
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Correspondence to Nou Ill-Sup.

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Hee-Jeong Jung and Nasar Uddin Ahmed have contributed equally to this work.

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Hee-Jeong, J., Nasar Uddin, A., Jong-In, P. et al. Analysis of S-locus and expression of S-alleles of self-compatible rapid-cycling Brassica oleracea ‘TO1000DH3’. Mol Biol Rep 41, 6441–6448 (2014). https://doi.org/10.1007/s11033-014-3526-6

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  • DOI: https://doi.org/10.1007/s11033-014-3526-6

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