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Differential expression analysis of a subset of GmNAC genes in shoots of two contrasting drought-responsive soybean cultivars DT51 and MTD720 under normal and drought conditions

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Abstract

NAC transcription factors are known to be involved in regulation of plant responses to drought stress. In this study, the expression of 23 drought-responsive GmNAC genes was assessed in the shoot tissues of DT51 and MTD720, the two soybean varieties with contrasting drought-responsive phenotypes, by real-time quantitative PCR (RT-qPCR) under normal and drought conditions. Results indicated that expression profile of GmNAC genes was genotype-dependent, and six GmNACs (GmNAC019, 043, 062, 085, 095 and 101) had higher transcript levels in the shoots of the drought-tolerant DT51 in comparison with the drought-sensitive MTD720 under drought. Our study suggests a positive correlation between the higher drought tolerance degree of DT51 versus MTD720 and the up-regulation of at least these six drought-responsive GmNACs in the shoot tissues. Furthermore, on the basis of our analysis, three genes, GmNAC043, 085 and 101, were identified as promising candidates for development of drought-tolerant soybean cultivars by genetic engineering.

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Acknowledgments

We would like to thank Dr. Tran Thi Truong from Vietnam Legumes Research and Development Center, and Dr. Nguyen Phuoc Dang from Can Tho University for providing seeds of various soybean cultivars. This research is funded by Vietnam National Foundation for Science and Technology Development (NAFOSTED) under grant number 106.16-2011.37 to Nguyen Phuong Thao.

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Authors and Affiliations

  1. School of Biotechnology, International University, Vietnam National University HCMC, Block 6, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam

    Nguyen Binh Anh Thu, Xuan Lan Thi Hoang, Hieu Doan, Dao Bui & Nguyen Phuong Thao

  2. Department of Plant Biotechnology, Faculty of Biology, University of Sciences, Vietnam National University HCMC, 227 Nguyen Van Cu Street, District 5, Ho Chi Minh City, Vietnam

    Thanh-Hao Nguyen

  3. Laboratoire de Biochimie et Physiologie Moléculaire des Plantes, CNRS/ENSAM/INRA/UM II, UMR 5004, 2 Place Viala, 34060, Montpellier Cedex 1, France

    Thanh-Hao Nguyen

  4. Signaling Pathway Research Unit, RIKEN Center for Sustainable Resource Science, 1-7-22, Suehiro-cho, Tsurumi, Yokohama, 230-0045, Japan

    Lam-Son Phan Tran

Authors
  1. Nguyen Binh Anh Thu
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  2. Xuan Lan Thi Hoang
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  3. Hieu Doan
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  4. Thanh-Hao Nguyen
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  5. Dao Bui
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  6. Nguyen Phuong Thao
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  7. Lam-Son Phan Tran
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Correspondence to Nguyen Phuong Thao or Lam-Son Phan Tran.

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Thu, N.B.A., Hoang, X.L.T., Doan, H. et al. Differential expression analysis of a subset of GmNAC genes in shoots of two contrasting drought-responsive soybean cultivars DT51 and MTD720 under normal and drought conditions. Mol Biol Rep 41, 5563–5569 (2014). https://doi.org/10.1007/s11033-014-3507-9

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  • DOI: https://doi.org/10.1007/s11033-014-3507-9

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