Skip to main content

Advertisement

SpringerLink
Log in

Matrilin-3 as a putative effector of C-type natriuretic peptide signaling during TGF-β induced chondrogenic differentiation of mesenchymal stem cells

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

C-type natriuretic peptide (CNP) signaling has been implicated as an important regulator of chondrogenic differentiation during endochondral bone development. This preliminary study further investigated the putative effectors and/or targets of CNP signaling in transforming growth factor (TGF)-β induced in vitro chondrogenic differentiation of mesenchymal stem cells (MSCs). Previously characterized human trabecular bone derived MSCs were induced either with only TGF-β1 or with a combination of TGF-β1 and CNP in micromass culture for 10 or 20 days. Genome wide gene expression profile changes in between these two groups were analyzed on day-10 or day-20 of culture. Results revealed that there were only 7 genes, whose expression change was fourfolds or higher in TGF-β1 and CNP fed group in comparison to only TGF-β1 fed group. The up-regulated genes included matrilin-3 (MATN3), engulfment and cell motility 1 (ELMO1), CD24, and DCN1, defective in cullin neddylation 1, domain containing 1 (DCUN1D1). The down-regulated genes, on the other hand, included LIM domain kinase 2 (LIMK2), Ewing sarcoma breakpoint region 1, and guanine nucleotide binding protein (G protein), gamma 12 (GNG12). The up-regulation of MATN3 was confirmed on the basis of RT-PCR. The known literature on both CNP signaling and MATN3 function in chondrogenesis match with each other and suggest MATN3 as a putative effector and/or target of CNP signaling during this process.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

References

  1. Alan T, Tufan AC (2008) C-type natriuretic peptide regulation of limb mesenchymal chondrogenesis is accompanied by altered N-cadherin and collagen type X-related functions. J Cell Biochem 105:227–235

    Article  CAS  PubMed  Google Scholar 

  2. Bartels CF, Bukulmez H, Padayatti P, Rhee DK, van Ravenswaaij-Arts C, Pauli RM, Mundlos S, Chitayat D, Shih LY, Al-Gazali LI, Kant S, Cole T, Morton J, Cormier-Daire V, Faivre L, Lees M, Kirk J, Mortier GR, Leroy J, Zabel B, Kim CA, Crow Y, Braverman NE, van den Akker F, Warman ML (2004) Mutations in the transmembrane natriuretic peptide receptor NPR-B impair skeletal growth and cause acromesomelic dysplasia, type Maroteaux. Am J Hum Genet 75:27–34

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  3. Chen Q, Johnson DM, Haudenschild DR, Tondravi MM, Goetinck PF (1995) Cartilage matrix protein forms a type II collagen-independent filamentous network: analysis in primary cell cultures with a retrovirus expression system. Mol Biol Cell 6:1743–1753

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  4. Fresquet M, Jowitt TA, Ylöstalo J, Coffey P, Meadows RS, Ala-Kokko L, Thornton DJ, Briggs MD (2007) Structural and functional characterization of recombinant matrilin-3 A-domain and implications for human genetic bone diseases. J Biol Chem 282:34634–34643

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  5. Funari VA, Day A, Krakow D, Cohn ZA, Chen Z, Nelson SF, Cohn DH (2007) Cartilage-selective genes identified in genome-scale analysis of non-cartilage and cartilage gene expression. BMC Genom 8:165

    Article  Google Scholar 

  6. Irizarry RA, Hobbs B, Collin F, Beazer-Barclay YD, Antonellis KJ, Scherf U, Speed TP (2003) Exploration, normalization, and summaries of high density oligonucleotide array probe level data. Biostatistics 4:249–264

  7. Kim OH, Park H, Seong MW, Cho TJ, Nishimura G, Superti-Furga A, Unger S, Ikegawa S, Choi IH, Song HR, Kim HW, Yoo WJ, Shim JS, Chung CY, Oh CW, Jeong C, Song KS, Seo SG, Cho SI, Yeo IK, Kim SY, Park S, Park SS (2011) Revisit of multiple epiphyseal dysplasia: ethnic difference in genotypes and comparison of radiographic features linked to the COMP and MATN3 genes. Am J Med Genet A 155A:2669–2680

    Article  PubMed  Google Scholar 

  8. Klatt AR, Becker AK, Neacsu CD, Paulsson M, Wagener R (2011) The matrilins: modulators of extracellular matrix assembly. Int J Biochem Cell Biol 43:320–330

    Article  CAS  PubMed  Google Scholar 

  9. Kocamaz E, Gok D, Cetinkaya A, Tufan AC (2012) Implication of C-type natriuretic peptide-3 signaling in glycosaminoglycan synthesis and chondrocyte hypertrophy during TGF-β1 induced chondrogenic differentiation of chicken bone marrow-derived mesenchymal stem cells. J Mol Histol 43:497–508

    Article  CAS  PubMed  Google Scholar 

  10. Lev R, Spicer S (1964) Specific staining of sulfated groups with Alcian blue at low pH. J Histochem Cytochem 12:309

    Article  CAS  PubMed  Google Scholar 

  11. Makihira S, Yan W, Ohno S, Kawamoto T, Fujimoto K, Okimura A, Yoshida E, Noshiro M, Hamada T, Kato Y (1999) Enhancement of cell adhesion and spreading by a cartilage-specific noncollagenous protein, cartilage matrix protein (CMP/Matrilin-1), via integrin alpha1beta1. J Biol Chem 274:11417–11423

    Article  CAS  PubMed  Google Scholar 

  12. Olney RC, Bukulmez H, Bartels CF, Prickett TC, Espiner EA, Potter LR, Warman ML (2006) Heterozygous mutations in natriuretic peptide receptor-B (NPR2) are associated with short stature. J Clin Endocrinol Metab 91:1229–1232

    Article  CAS  PubMed  Google Scholar 

  13. Pei M, Luo J, Chen Q (2008) Enhancing and maintaining chondrogenesis of synovial fibroblasts by cartilage extracellular matrix protein matrilins. Osteoarthr Cartil 16:1110–1117

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  14. Potter LR, Abbey-Hosch S, Dickey DM (2006) Natriuretic peptides, their receptors, and cyclic guanosine monophosphate-dependent signaling functions. Endocr Rev 27:47–72

    Article  CAS  PubMed  Google Scholar 

  15. Tezcan B, Serter S, Kiter E, Tufan AC (2010) Dose dependent effect of C-type natriuretic peptide signaling in glycosaminoglycan synthesis during TGF-b1 induced chondrogenic differentiation of mesenchymal stem cells. J Mol Histol 41:247–258

    Article  CAS  PubMed  Google Scholar 

  16. Tuli R, Tuli S, Nandi S, Wang ML, Alexander PG, Haleem-Smith H, Hozack WJ, Manner PA, Danielson KG, Tuan RS (2003) Characterization of multipotential mesenchymal progenitor cells derived from human trabecular bone. Stem Cells 21:681–693

    Article  CAS  PubMed  Google Scholar 

  17. Wiberg C, Klatt AR, Wagener R, Paulsson M, Bateman JF, Heinegård D, Mörgelin M (2003) Complexes of matrilin-1 and biglycan or decorin connect collagen VI microfibrils to both collagen II and aggrecan. J Biol Chem 278:37698–37704

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by funding from TUBITAK, The Scientific and Technological Research Council of Turkey (Grant: SBAG-108S189). Authors thank Professor Hilal Ozdag, from Biotechnology Institute, Ankara University, Ankara, Turkey, for her generous guidance in microarray preprocessing, quality control, differentially expressed gene set analyses, sample based clustering analyses, and bioinformatics.

Author information

Author notes

  1. Berna Tezcan

    Present address: Department of Histology and Embryology, School of Medicine, Giresun University, Giresun, Turkey

  2. A. Cevik Tufan

    Present address: Department of Histology and Embryology, School of Medicine, Yildirim Beyazit University, Ankara, Turkey

Authors and Affiliations

  1. Department of Biomedical Engineering, Faculty of Engineering and Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Canada

    Mustafa Ege Babadagli

  2. Department of Electrical and Computer Engineering, Faculty of Engineering, University of Alberta, Edmonton, Canada

    Mustafa Ege Babadagli

  3. Department of Histology and Embryology, School of Medicine, Osman Gazi University, Eskisehir, Turkey

    Berna Tezcan

  4. Biotechnology Institute, Ankara University, Ankara, Turkey

    Seda Tasir Yilmaz

  5. Department of Histology and Embryology, School of Medicine, Pamukkale University, Denizli, Turkey

    A. Cevik Tufan

Authors
  1. Mustafa Ege Babadagli
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Berna Tezcan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Seda Tasir Yilmaz
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. Cevik Tufan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. Cevik Tufan.

Additional information

Mustafa Ege Babadagli participated as a voluntary summer student in this study.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Babadagli, M.E., Tezcan, B., Yilmaz, S.T. et al. Matrilin-3 as a putative effector of C-type natriuretic peptide signaling during TGF-β induced chondrogenic differentiation of mesenchymal stem cells. Mol Biol Rep 41, 5549–5555 (2014). https://doi.org/10.1007/s11033-014-3448-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-014-3448-3

Keywords

Search

Navigation