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Microsatellite markers for Lycium ruthenicum (Solananeae)

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Abstract

We developed microsatellite markers in Lycium ruthenicum, a desert plant widely distributed in northwestern China. In order to investigate its population genetic structure, genetic diversity, and its evolutionary history, we have isolated 11 novel microsatellite loci primers and characterized them in 24 individuals from 3 populations of L. ruthenicum using the combined biotin capture technique. For these microsatellites, one to seven alleles per locus were identified. The observed heterozygosities ranged from 0 to 0.958, meanwhile the expected heterozygosities ranged from 0 to 0.841. These microsatellite markers could be first useful for population level studies like genetic diversity and structure in this species.

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Acknowledgments

This work was supported by the development of Beifang University of Nationnalities for the National Natural Science Foundation of China (2012QZP09).

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Authors and Affiliations

  1. School of Life Sciences, Fudan University, Shanghai, 200433, China

    Hai-kui Chen & Yang Zhong

  2. Department of Life Science, Beifang University of Nationalities, Yinchuan, 750021, China

    Hai-kui Chen

  3. Institute of Biodiversity Science and Geobiology, Tibet University, Lhasa, 850000, China

    Yang Zhong

Authors
  1. Hai-kui Chen
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  2. Yang Zhong
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Correspondence to Yang Zhong.

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Chen, Hk., Zhong, Y. Microsatellite markers for Lycium ruthenicum (Solananeae). Mol Biol Rep 41, 5545–5548 (2014). https://doi.org/10.1007/s11033-014-3442-9

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  • DOI: https://doi.org/10.1007/s11033-014-3442-9

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