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A murrel interferon regulatory factor-1: molecular characterization, gene expression and cell protection activity

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Abstract

In this study, we have reported a first murrel interferon regulatory factor-1 (designated as Murrel IRF-1) which is identified from a constructed cDNA library of striped murrel Channa striatus. The identified sequence was obtained by internal sequencing method from the library. The Murrel IRF-1 varies in size of the polypeptide from the earlier reported fish IRF-1. It contains a DNA binding domain along with a tryptophan pentad repeats, a nuclear localization signal and a transactivation domain. The homologous analysis showed that the Murrel IRF-1 had a significant sequence similarity with other known fish IRF-1 groups. The phylogenetic analysis exhibited that the Murrel IRF-1 clustered together with IRF-1 members, but the other members including IRF-2, 3, 4, 5, 6, 7, 8, 9 and 10 were clustered individually. The secondary structure of Murrel IRF-1 contains 27 % α-helices (85 aa residues), 5.7 % β-sheets (19 aa residues) and 67.19 % random coils (210 aa residues). Furthermore, we predicted a tertiary structure of Murrel IRF-1 using I-Tasser program and analyzed the structure on PyMol surface view. The RNA structure of the Murrel IRF-1 along with its minimum free energy (−284.43 kcal/mol) was also predicted. The highest gene expression was observed in spleen and its expression was inducted with pathogenic microbes which cause epizootic ulcerative syndrome in murrels such as fungus, Aphanomyces invadans and bacteria, Aeromonas hydrophila, and poly I:C, a viral RNA analog. The results of cell protection assay suggested that the Murrel IRF-1 regulates the early defense response in C. striatus. Moreover, it showed Murrel IRF-1 as a potential candidate which can be developed as a therapeutic agent to control microbial infections in striped murrel. Overall, these results indicate the immune importance of IRF-1, however, the interferon signaling mechanism in murrels upon infection is yet to be studied at proteomic level.

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Acknowledgments

This research is supported by DBT’s Prestigious Ramalingaswami Re-entry Fellowship (D.O.NO.BT/HRD/35/02/2006) funded by Department of Biotechnology, Ministry of Science and Technology, Government of India, New Delhi.

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Authors and Affiliations

  1. Division of Fisheries Biotechnology & Molecular Biology, Department of Biotechnology, Faculty of Science and Humanities, SRM University, Kattankulathur, Chennai, 603 203, Tamil Nadu, India

    Jesu Arockiaraj, Akila Sathyamoorthi, Venkatesh Kumaresan, Rajesh Palanisamy, Mukesh Kumar Chaurasia, Prasanth Bhatt & Abirami Arasu

  2. Department of Biotechnology, SRM Arts & Science College, Kattankulathur, Chennai, 603 203, India

    Akila Sathyamoorthi

  3. Institute for Cellular and Molecular Biology, The University of Texas at Austin, 1 University Station A4800, Austin, TX, 78712, USA

    Annie J. Gnanam

  4. Lab PCN 206, Microbiology Division, CSIR-Central Drug Research Institute, B.S. 10/1, Sector 10 Jankipuram Extension, Sitapur Road, Lucknow, 226031, Uttar Pradesh, India

    Mukesh Pasupuleti

  5. Department of Microbiology, SRM Arts & Science College, Kattankulathur, Chennai, 603 203, India

    Abirami Arasu

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  1. Jesu Arockiaraj
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Correspondence to Jesu Arockiaraj.

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Arockiaraj, J., Sathyamoorthi, A., Kumaresan, V. et al. A murrel interferon regulatory factor-1: molecular characterization, gene expression and cell protection activity. Mol Biol Rep 41, 5299–5309 (2014). https://doi.org/10.1007/s11033-014-3401-5

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