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Identification and characterization of six cytochrome P450 genes belonging to CYP4 and CYP6 gene families in the silkworm, Bombyx mori

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Abstract

It was predicted that the genome of silkworm, Bombyx mori, has at least 79 P450 genes; however, P450 genes that are related to the catabolism of exogenous compounds were not reported. In this study we cloned two CYP4 (named CPY4M5 and CYP4M9) and four CYP6 (named CYP6AB5, CYP6AE9, CYP6AE22 and CYP6AU1) genes by using both bioinformatics and RT-PCR approaches. Sequence analysis showed that these genes contained conserved P450 gene sequence regions and one conserved intron. CYP4M5 and CYP4M9 genes were clustered together in a mode of “head-to-tail” possibly due to gene duplication. Blast analysis showed that these P450 genes shared significant similarity with CYP4 and CYP6 genes that are involved in the catabolism and detoxification of exogenous compounds in other insect species. RT-PCR results showed that these P450 genes were highly expressed in the midgut and fat body of B. mori. As the instar age increased, these P450 genes exhibit different expression patterns. When B. mori was exposed to 1.75 × 10−5 % of cypermethrin, 3.5 × 10−6 % of cypermethrin and 0.1 % of rutin, expression of CYP6AB5 was increased by 2.3-fold, 2.2-fold and 1.9-fold, respectively. Exposure of B. mori to 0.1 % quercetin does not change the expression of CYP6AB5. In contrast, expression of the other five P450 genes was inhibited after exposed to these compounds.

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Acknowledgments

This work was supported by the National High Technology Research and Development Program of China (863 Program) (Grant No. 2013AA102507), the State Key Laboratory of Silkworm Genome Biology, the transformation project of agriculture scientific and technological achievements (2013GB2C100180), the Priority Academic Program Development of Jiangsu Higher Education Institutions, the Doctoral Fund of Ministry of Education of China (20113201110008), the China Agriculture Research System (CARS-22-ZJ0305), and the Science & Technology support Program of Suzhou (ZXS2012005, SNG201352).

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Authors and Affiliations

  1. School of Basic Medicine and biological sciences, Soochow University, Suzhou, 215123, Jiangsu, China

    Bing Li, Hua Zhang, Min Ni, Bin-bin Wang, Fan-chi Li, Kai-zhun Xu & Wei-de Shen

  2. State Key Laboratory of Silkworm Genome Biology, Chongqing, 400716, China

    Bing Li, Qing-you Xia & Ping Zhao

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  1. Bing Li
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  2. Hua Zhang
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Correspondence to Bing Li or Ping Zhao.

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Bing Li, Hua Zhang and Min Ni have contributed equally to the study.

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Li, B., Zhang, H., Ni, M. et al. Identification and characterization of six cytochrome P450 genes belonging to CYP4 and CYP6 gene families in the silkworm, Bombyx mori . Mol Biol Rep 41, 5135–5146 (2014). https://doi.org/10.1007/s11033-014-3379-z

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