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Molecular cloning, characterization and expression analysis of Melanotransferrin from the sea cucumber Apostichopus japonicus

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Abstract

Melanotransferrin (MTf), a member of the transferrin families, plays an important role in immune response. But the research about MTf in sea cucumber is limited till now. In this study, the Melanotransferrin (Aj-MTf) gene was firstly cloned and characterized from the sea cucumber Apostichoupus japonicus by reverse transcriptase polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends. The full-length cDNA of Aj-MTf is 2,840 bp in length and contains a 2,184 bp open reading frame that encodes a polypeptide of 727 amino acids. An iron-responsive element-like structure is located at the 5′-UTR of Aj-MTf cDNA. Sequence analysis shows that the Aj-MTf contains two conserved domains, and the binding-iron (III) sites, including eight amino acid residues (D81,Y109,Y215,H283,D425,Y454,Y565 and H634) and three N-linked glycosylation sites (N121V122S123,N173A174S175 and N673S674T675). Quantitative real-time polymerase chain reaction (qRT-PCR) analyses suggested that the Aj-MTf expressions in the coelomic fluid, body cavity wall and respiratory trees were significantly changed from 4 to 24 h post lipopolysaccharide (LPS) injection. The mRNA levels of Aj-MTf in coelomic fluid was significantly up-regulated at 12 and 24 h in treatment group, and Aj-MTf shared a similar expression pattern with C-type lectin in coelomic fluid, while both genes appears to gradually increase after 4 h of LPS injection. These results indicate that the Aj-MTf plays a pivotal role in immune responses to the LPS challenge in sea cucumber, and provide new information that it is complementary to the sea cucumber immune genes and initiate new researches concerning the genetic basis of the holothurian immune response.

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Acknowledgments

We thank Master Shixin Liu (College of Foreign Languages, Dalian Ocean University, Dalian, China) for his help in revising the manuscript. This project was supported by the Key Laboratory Foundation of the Educational Department of Liaoning Province of China (Grant No.2009S024).

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  1. College of Fisheries and Life Science, Dalian Ocean University, 52 Heishijiao Street, Shahekou District, Dalian, 116023, China

    Xuemei Qiu, Dong Li, Jun Cui, Yang Liu & Xiuli Wang

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  1. Xuemei Qiu
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  2. Dong Li
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  3. Jun Cui
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  5. Xiuli Wang
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Correspondence to Xiuli Wang.

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Qiu, X., Li, D., Cui, J. et al. Molecular cloning, characterization and expression analysis of Melanotransferrin from the sea cucumber Apostichopus japonicus . Mol Biol Rep 41, 3781–3791 (2014). https://doi.org/10.1007/s11033-014-3243-1

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  • DOI: https://doi.org/10.1007/s11033-014-3243-1

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