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Association between cytokines and methylation of SOCS-1 in serum of patients with ankylosing spondylitis

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Abstract

In this study, we aim to determine the relationship between methylation level of an inflammatory-related gene, SOCS-1 in serum samples of patients with ankylosing spondylitis (AS) and their degree of inflammation as well as serum cytokine level. Quantitative real time methylation specific PCR was performed to examine the promoter methylation of SOCS-1 in serum samples of 43 HLA-B27+ AS patients and 6 B27+ healthy controls. Degree of inflammation was accessed by spondylopathy, sacroiliitis as well as acute phase reactant, erythrocyte sedimentation rate and C-reactive protein (CRP). Serum IL-6 and TNF-α level was determined by ELISA assay. SOCS-1 methylation can only be found in serums samples from patients but not normal control. Methylation of SOCS-1 significantly associated with severity of patient’s spondylopathy (P < 0.005), sacroiliitis (P < 0.005) and acute phase reactant CRP (P = 0.0278). AS patients also exhibited higher serum IL-6 (P < 0.001) and TNF-α level (P < 0.001). Importantly, patients with high serum IL-6 or TNF-α level demonstrated a significantly higher SOCS-1 methylation (P < 0.001). In conclusion, this proof-of-principle study suggested that methylation of SOCS-1 can be detected in serum of HLA-B27+ AS patients but not in B27+ controls. The pathogenic potential of SOCS-1 methylation in AS deserves further investigation.

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Acknowledgments

This study was supported by research Grant from Dalin Tzu Chi General Hospital (98-2-2); College of Science, National Chung Cheng University and National Science Council, Taiwan (NSC100-2320-B-194-002).

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The authors have declared no conflicts of interest

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Authors and Affiliations

  1. Department of Allergy, Immunology and Rheumatology, Buddhist Dalin Tzu Chi General Hospital, Dalin, Chia-Yi, Taiwan, ROC

    Ning-Sheng Lai, Su-Qin Liu & Ming-Chi Lu

  2. School of Medicine, Tzu Chi University, Hualin, Taiwan, ROC

    Ning-Sheng Lai, Su-Qin Liu & Ming-Chi Lu

  3. Department of Life Science, National Chung Cheng University, Room 452, 168 University Road, Min-Hsiung, Chia-Yi, 621, Taiwan, ROC

    Jian-Liang Chou, Gary C. W. Chen & Michael W. Y. Chan

  4. Human Epigenomics Center, National Chung Cheng University, Min-Hsiung, Chia-Yi, Taiwan, ROC

    Michael W. Y. Chan

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  1. Ning-Sheng Lai
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Correspondence to Michael W. Y. Chan.

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Lai, NS., Chou, JL., Chen, G.C.W. et al. Association between cytokines and methylation of SOCS-1 in serum of patients with ankylosing spondylitis. Mol Biol Rep 41, 3773–3780 (2014). https://doi.org/10.1007/s11033-014-3242-2

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  • DOI: https://doi.org/10.1007/s11033-014-3242-2

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