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An in-vitro investigation of the effect of perfluorooctane sulphonate on cell lines of embryonic origin

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Abstract

Fluorinated organic compounds, such as perfluorooctane sulfonate, are stable chemicals with a wide range of industrial applications. The potential toxicity of perfluorooctane sulfonate is not well characterized, and even less known are the mechanisms underlying its toxic effects. Perfluorooctane sulfonate change of inner mitochondrial membrane permeability has been implicated as a potential mechanism of toxicity. In this study, we research that perfluorooctane sulfonate effects the expression of Apaf1 and Caspase3 genes in the amnion and fetal lung cell line that initiate the cells to undergo apoptosis. The expression of Caspase3 and Apaf1 was determined by using quantitative RT-PCR. In the study there is significant increase in expression of Caspase3 and Apaf1 in amnion and fetal lung cell line exposed to high dose (p < 0.001, p = 0.004). Also there is significant increase in cell lines exposed for a long period of time to perfluorooctane sulfonate (p = 0.001). But no significant increase was seen in the low doses and exposed for a short period of time. In conclusion, apoptotic gene expression is increase in cells exposed perfluorooctane sulfonate by dose dependent manner was determined. So this work is the first study examines the apoptotic effects of perfluorooctane sulfonate in human embryonic cells it will lead the way to the other topical studies.

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References

  1. Olsen GW, Burris JM, Mandel JH, Zobel LR (1999) Serum perfluorooctane sulfonate and hepatic and lipid clinical chemistry tests in fluorochemical production employees. J Occup Environ Med 41(9):799–806

    Article  CAS  PubMed  Google Scholar 

  2. Meesters RJ, Schröder HF (2004) Perfluorooctane sulfonate—a quite mobile anionic anthropogenic surfactant, ubiquitously found in the environment. Water Sci Technol 50(5):235–242

    CAS  PubMed  Google Scholar 

  3. M Reports “The Science of organic fluorochemistry” and “perfluorooctane sulfonate: current summary of human sera, health and toxicology data”, 5 Feb 1999

  4. Sargent JW, Seffl RJ (1970) Properties of perfluorinated liquids. Fed Proc 29(5):1699–1703

    CAS  PubMed  Google Scholar 

  5. Hu W, Jones PD, Upham BL, Trosko JE, Lau C, Giesy JP (2002) Inhibition of gap junctional intercellular communication by perfluorinated compounds in rat liver and dolphin kidney epithelial cell lines in vitro and Sprague-Dawley rats in vivo. Toxicol Sci 68(2):429–436

    Article  CAS  PubMed  Google Scholar 

  6. Giesy JP, Kannan K (2001) Global distribution of perfluorooctane sulfonate in wildlife. Environ Sci Technol 35:1339–1342

    Article  CAS  PubMed  Google Scholar 

  7. Seacat AM, Thomford PJ, Hansen KJ, Olsen GW, Case MT, Butenhoff JL (2002) Subchronic toxicity studies on perfluorooctanesulfonate potassium salt in cynomolgus monkeys. Toxicol Sci 68:249–264

    Article  CAS  PubMed  Google Scholar 

  8. Kannan K, Corsolini S, Falandysz J, Fillmann G, Kumar KS, Loganathan BG, Mohd MA, Olivero J, Van Wouwe N, Yang JH, Aldoust KM (2004) Perfluorooctanesulfonate and related fluorochemicals in human blood from several countries. Environ Sci Technol 38(17):4489–4495

    Article  CAS  PubMed  Google Scholar 

  9. Harada K, Saito N, Inoue K, Yoshinaga T, Watanabe T, Sasaki S, Kamiyama S, Koizumi A (2004) The influence of time, sex and geographic factors on levels of perfluorooctane sulfonate and perfluorooctanoate in human serum over the last 25 years. J Occup Health 46(2):141–147

    Article  CAS  PubMed  Google Scholar 

  10. Shaw S, Berger ML, Brenner D, Tao L, Wu Q, Kannan K (2009) Specific accumulation of perfluorochemicals in harbor seals (Phoca vitulina concolor) from the northwest Atlantic. Chemosphere 74(8):1037–1043

    Article  CAS  PubMed  Google Scholar 

  11. Paiano V, Fattore E, Carrà A, Generoso C, Fanelli R, Bagnati R (2012) Liquid chromatography-tandem mass spectrometry analysis of perfluorooctane sulfonate and perfluorooctanoic acid in fish fillet samples. J Anal Methods Chem 2012:719010. doi:10.1155/2012/719010

    Article  PubMed Central  PubMed  Google Scholar 

  12. Grasty RC, Wolf DC, Grey BE, Lau CS, Rogers JM (2003) Prenatal window of susceptibility to perfluorooctane sulfonate-induced neonatal mortality in the Sprague-Dawley rat. Birth Defects Res B Dev Reprod Toxicol 68(6):465–471

    Article  CAS  PubMed  Google Scholar 

  13. Luebker DJ, Case MT, York RG, Moore JA, Hansen KJ, Butenhoff JL (2005) Two-generation reproduction and cross-foster studies of perfluorooctanesulfonate (PFOS) in rats. Toxicology 215(1–2):126–148

    Article  CAS  PubMed  Google Scholar 

  14. Lau C, Thibodeaux JR, Hanson RG, Rogers JM, Grey BE, Stanton ME, Butenhoff JL, Stevenson LA (2003) Exposure to perfluorooctane sulfonate during pregnancy in rat and mouse. II: postnatal evaluation. Toxicol Sci 74(2):382–392

    Article  CAS  PubMed  Google Scholar 

  15. Lau C, Butenhoff JL, Rogers JM (2004) The developmental toxicity of perfluoroalkyl acids and their derivatives. Toxicol Appl Pharmacol 198:231–241

    Article  CAS  PubMed  Google Scholar 

  16. Grasty RC, Bjork JA, Wallace KB, Wolf DC, Lau CS, Rogers JM (2005) Effects of prenatal perfluorooctane sulfonate (PFOS) exposure on lung maturation in the perinatal rat. Birth Defects Res B Dev Reprod Toxicol 74(5):405–416

    Article  CAS  PubMed  Google Scholar 

  17. Apelberg BJ, Witter FR, Herbstman JB, Calafat AM, Halden RU, Needham LL, Goldman LR (2007) Cord serum concentrations of perfluorooctane sulfonate (PFOS) and perfluorooctanoate (PFOA) in relation to weight and size at birth. Environ Health Perspect 115(11):1670–1676

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  18. Inoue K, Okada F, Ito R, Kato S, Sasaki S, Nakajima S, Uno A, Saijo Y, Sata F, Yoshimura Y, Kishi R, Nakazawa H (2004) Perfluorooctane sulfonate (PFOS) and related perfluorinated compounds in human maternal and cord blood samples: assessment of PFOS exposure in a susceptible population during pregnancy. Environ Health Perspect 112(11):1204–1207

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  19. Panaretakis T, Shabalina IG, Grandér D, Shoshan MC, DePierre JW (2001) Reactive oxygen species and mitochondria mediate the induction of apoptosis in human hepatoma HepG2 cells by the rodent peroxisome proliferator and hepatocarcinogen, perfluorooctanoic acid. Toxicol Appl Pharmacol 173(1):56–64

    Article  CAS  PubMed  Google Scholar 

  20. O’Brien TM, Wallace KB (2004) Mitochondrial permeability transition as the critical target of N-acetyl perfluorooctane sulfonamide toxicity in vitro. Toxicol Sci 82(1):333–340

    Article  PubMed  Google Scholar 

  21. Estaquier J, Vallette F, Vayssiere JL, Mignotte B (2012) The mitochondrial pathways of apoptosis. Adv Exp Med Biol 942:157–183

    Article  CAS  PubMed  Google Scholar 

  22. Hu XZ, Hu DC (2009) Effects of perfluorooctanoate and perfluorooctane sulfonate exposure on hepatoma HepG2 cells. Arch Toxicol 83(9):851–861

    Article  CAS  PubMed  Google Scholar 

  23. Liu C, Yu K, Shi X, Wang J, Lam PK, Wu RS, Zhou B (2007) Induction of oxidative stress and apoptosis by PFOS and PFOA in primary cultured hepatocytes of freshwater tilapia (Oreochromis niloticus). Aquat Toxicol 82(2):135–143

    Article  CAS  PubMed  Google Scholar 

  24. Watanabe MX, Jones SP, Iwata H, Kim EY, Kennedy SW (2009) Effects of co-exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin and perfluorooctane sulfonate or perfluorooctanoic acid on expression of cytochrome P450 isoforms in chicken (Gallus gallus) embryo hepatocyte cultures. Comp Biochem Physiol C 149(4):605–612

    Google Scholar 

  25. Shi X, Du Y, Lam PK, Wu RS, Zhou B (2008) Developmental toxicity and alteration of gene expression in zebrafish embryos exposed to PFOS. Toxicol Appl Pharmacol 230(1):23–32

    Article  CAS  PubMed  Google Scholar 

  26. Krovel AV, Softeland L, Torstensen B, Olsvik PA (2008) Transcriptional effects of PFOS in isolated hepatocytes from Atlantic salmon Salmo salar L. Comp Biochem Physiol C 148(1):14–22

    Google Scholar 

  27. Thibodeaux JR, Hanson RG, Rogers JM, Grey BE, Barbee BD, Richards JH, Butenhoff JL, Stevenson LA, Lau C (2003) Exposure to perfluorooctane sulfonate during pregnancy in rat and mouse I: maternal and prenatal evaluations. Toxicol Sci 74(2):369–381

    Article  CAS  PubMed  Google Scholar 

  28. Starkov AA, Wallace KB (2002) Structural determinants of fluorochemical-induced mitochondrial dysfunction. Toxicol Sci 66(2):244–252

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Medical Biology, Faculty of Medicine, Bulent Ecevit University, Zonguldak, Turkey

    Sevim Karakas-Celik

  2. Department of Medical Biology and Genetic, Faculty of Medicine, Mersin University, Mersin, Turkey

    Nurcan Aras

Authors
  1. Sevim Karakas-Celik
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  2. Nurcan Aras
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Correspondence to Sevim Karakas-Celik.

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Karakas-Celik, S., Aras, N. An in-vitro investigation of the effect of perfluorooctane sulphonate on cell lines of embryonic origin. Mol Biol Rep 41, 3755–3759 (2014). https://doi.org/10.1007/s11033-014-3240-4

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  • DOI: https://doi.org/10.1007/s11033-014-3240-4

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