Molecular Biology Reports

, Volume 41, Issue 5, pp 2697–2707 | Cite as

Conservation and function of Dazl in promoting the meiosis of goat male germline stem cells

  • Zhiwei Niu
  • Yue Hu
  • Mingzhi Liao
  • Meng Yu
  • Haijing Zhu
  • Long Wang
  • Jiang Wu
  • Chunling Bai
  • Guangpeng Li
  • Jinlian Hua
Article

Abstract

Dazl (deleted in azoospermia-like) is a conserved gene in mammalian meiosis, which encodes RNA binding protein required for spermatocyte meiosis. Up to date, the expression and function of Dazl in the goat testis are unknown. The objectives of this study were to investigate the expression pattern of Dazl in dairy goat testis and their function in male germline stem cells (mGSCs). The results first revealed that the expression level of Dazl in adult testes was significantly higher than younger and immature goats, and azoospermia and male intersex testis. The dairy goat Dazl is highly conserved analysed by several online and bioinformatics software, respectively. Over-expression of Dazl promoted the expression of meiosis-related genes in dairy goat mGSCs. The expression of Stra8 was up-regulated by over-expression of Dazl analysed by Luciferase reporter assay. Taken together, results suggest the Dazl plays an important role in dairy goat spermatogenesis and that over-expression of Dazl may promote Stra8 expression in dairy goat mGSCs.

Keywords

Dazl Meiosis Spermatogenesis Male germline stem cells (mGSCs) Dairy goat 

Supplementary material

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Zhiwei Niu
    • 1
  • Yue Hu
    • 1
  • Mingzhi Liao
    • 3
  • Meng Yu
    • 1
  • Haijing Zhu
    • 1
  • Long Wang
    • 1
  • Jiang Wu
    • 1
  • Chunling Bai
    • 2
  • Guangpeng Li
    • 2
  • Jinlian Hua
    • 1
  1. 1.College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Key Lab for Animal Biotechnology of Agriculture Ministry of ChinaNorthwest A&F UniversityYanglingChina
  2. 2.Key Laboratory for Mammalian Reproductive Biology and Biotechnology, Ministry of EducationInner Mongolia UniversityHohhotChina
  3. 3.3, College of Life ScienceNorthwest A&F UniversityYanglingChina

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