Conservation and function of Dazl in promoting the meiosis of goat male germline stem cells
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Dazl (deleted in azoospermia-like) is a conserved gene in mammalian meiosis, which encodes RNA binding protein required for spermatocyte meiosis. Up to date, the expression and function of Dazl in the goat testis are unknown. The objectives of this study were to investigate the expression pattern of Dazl in dairy goat testis and their function in male germline stem cells (mGSCs). The results first revealed that the expression level of Dazl in adult testes was significantly higher than younger and immature goats, and azoospermia and male intersex testis. The dairy goat Dazl is highly conserved analysed by several online and bioinformatics software, respectively. Over-expression of Dazl promoted the expression of meiosis-related genes in dairy goat mGSCs. The expression of Stra8 was up-regulated by over-expression of Dazl analysed by Luciferase reporter assay. Taken together, results suggest the Dazl plays an important role in dairy goat spermatogenesis and that over-expression of Dazl may promote Stra8 expression in dairy goat mGSCs.
KeywordsDazl Meiosis Spermatogenesis Male germline stem cells (mGSCs) Dairy goat
This work was supported by the Grants from the Program (31272518, 30972097) of National Natural Science Foundation of China, Doctoral Fund of Ministry of Education of China (RFDP, 20120204110030), The authors appreciate every member in the Shaanxi Centre of Stem Cells Engineering & Technology for their help.
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