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MiR-320a down-regulation mediates bladder carcinoma invasion by targeting ITGB3

Molecular Biology Reports volume 41, pages 2521–2527 (2014)Cite this article

Abstract

To investigate whether the miR-320a could regulate bladder cancer cells invasion by down-regulation of ITGB3. Real-time quantitative PCR was applied to evaluate the expression level of miRNA-320a in bladder transitional cell carcinomas (TCC) and normal bladder transitional cell (NBTC) samples. The invasion ability of miR-320a in TCC T24 cells was analyzed by Transwell assay after pre-miR-320a or anti-miR-125b transfection. For the invasion mechanism analysis of miR-320a on T24 cells, TargetScan, PicTar and miRBase were used to predict the possible target gene of miR-320a. Luciferase activities assay and western blot were used to reveal the predicted target gene of miR-320a were direct and specific. RNA interference technology was used to confirm the invasion inhibition of miR-320a was directly induced by ITGB3. Our study showed that miR-320a was down-regulated in human TCC specimens compared to that in NBTC specimens. Over-expression of miR-320a in T24 cells inhibited TCC invasion and this inhibitory effect on T24 cells could be restore by miR-320a knocked down. Mechanism analysis revealed that ITGB3 was a direct and specific target of miR-320a. The advanced effect of anti-miR-320a on TCC cell invasion was mediated by expression silence of ITGB3. In summary, aberrantly expressed miR-320a contribute to T24 cells invasion partly through directly down-regulating ITGB3 protein expression in TCC and this miRNA signature offers a novel potential therapeutic strategy for TCC.

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Acknowledgments

This work was supported by the National Nature Science Foundation of China (81172408, 30901480, 81301834, 81272716, 81301862).

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Affiliations

  1. Department of Neurobiology, China Medical University, Shenyang, 110001, People’s Republic of China

    Chao Shang & Yixue Xue

  2. Institute of Pathology and Pathophysiology, China Medical University, Shenyang, People’s Republic of China

    Chao Shang & Yixue Xue

  3. Department of Urinary Surgery, The Affiliated Shengjing Hospital, China Medical University, Shenyang, 110004, People’s Republic of China

    Hui Zhang

  4. Department of Central Laboratory, School of Stomatology, China Medical University, Shenyang, 110007, People’s Republic of China

    Yan Guo

  5. Department of Neurosurgery, The Affiliated Shengjing Hospital, China Medical University, Shenyang, 110004, People’s Republic of China

    Yang Hong & Yunhui Liu

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  1. Chao Shang
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  2. Hui Zhang
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  3. Yan Guo
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  4. Yang Hong
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  5. Yunhui Liu
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  6. Yixue Xue
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Correspondence to Yixue Xue.

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Shang, C., Zhang, H., Guo, Y. et al. MiR-320a down-regulation mediates bladder carcinoma invasion by targeting ITGB3. Mol Biol Rep 41, 2521–2527 (2014). https://doi.org/10.1007/s11033-014-3110-0

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  • DOI: https://doi.org/10.1007/s11033-014-3110-0

Keywords

  • Bladder carcinoma
  • MiR-320a
  • ITGB3
  • Invasion