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Molecular cloning and characterization of 5-enolpyruvylshikimate-3-phosphate synthase gene from Convolvulus arvensis L.

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Abstract

5-Enolpyruvylshikimate-3-phosphate synthase (EPSPS), the target enzyme for glyphosate inhibition, catalyzes an essential step in the shikimate pathway for aromatic amino acid biosynthesis. The full-length cDNA of 1,751 nucleotides (CaEPSPS, Genbank accession number: EU698030) from Convolvulus arvensis was cloned and characterized. The CaEPSPS encodes a polypeptide of 520 amino acids with a calculated molecular weight of 55.5 kDa and an isoelectric point of 7.05. The results of homology analysis revealed that CaEPSPS showed highly homologous with EPSPS proteins from other plant species. Tissue expression pattern analysis indicated that CaEPSPS was constitutively expressed in stems, leaves and roots, with lower expression in roots. CaEPSPS expression level could increase significantly with glyphosate treatment, and reached its maximum at 24 h after glyphosate application. We fused CaEPSPS to the CaMV 35S promoter and introduced the chimeric gene into Arabidopsis. The resultant expression of CaEPSPS in transgenic Arabidopsis plants exhibited enhanced tolerance to glyphosate in comparison with control.

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Acknowledgments

We would like to thank Yuehui Chao from Institute of Animal Sciences, CAAS for assistance with transgenic plant culture and testing. We also appreciate the constructive and helpful comments on the manuscript from the editor and the anonymous reviewers.

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Authors and Affiliations

  1. Institute of Plant Protection, Key Laboratory of Weed and Rodent Biology and Management, Chinese Academy of Agricultural Sciences, Beijing, 100193, China

    Zhao-Feng Huang, Chao-Xian Zhang, Hong-Juan Huang, Shou-Hui Wei, Hai-Lan Cui, Jing-Chao Chen, Long Yang & Jin-Yi Chen

  2. Institute of Plant Protection, Heilongjiang Academy of Land Reclamation Sciences, Harbin, 150038, China

    Yan Liu

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  1. Zhao-Feng Huang
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Correspondence to Chao-Xian Zhang.

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Huang, ZF., Zhang, CX., Huang, HJ. et al. Molecular cloning and characterization of 5-enolpyruvylshikimate-3-phosphate synthase gene from Convolvulus arvensis L.. Mol Biol Rep 41, 2077–2084 (2014). https://doi.org/10.1007/s11033-014-3056-2

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  • DOI: https://doi.org/10.1007/s11033-014-3056-2

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