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Cyclooxygenase-2 single-nucleotide polymorphisms and hepatocellular carcinoma in Egypt

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Abstract

Hepatocellular carcinoma (HCC) is a worldwide neoplasm for which early diagnosis is difficult and the prognosis is usually poor. Overexpression of cyclooxygenase 2 (COX-2) has been suggested to be associated with hepatocarcinogenesis. Although several COX-2 inhibitors have been used in hepatoma therapy, the genetic background between COX-2 and HCC remains largely unknown. In this study, the association of genotypic polymorphisms in COX-2 with HCC was investigated. 25 healthy individuals served as control (group I), group II: 50 HCV infection patients without any complications, group III: 50 HCV infected patients complicated with cirrhosis and group IV: 75 HCV infected patients complicated with (45 localized and 30 metastatic) HCC from Zagazig University Hospital in Egypt were genotyped by a PCR–RFLP method. The results showed that, no differences in distribution between the HCC and other groups were found. We found −1195A allele carriers had a higher risk of HCC with HCV infection. As regard the obtained results of serum AFU, a significant increase was detected in HCC as compared with cirrhosis, hepatitis and healthy control groups (p < 0.001). Concerning the obtained results of serum AFP, when HCC group was compared with cirrhosis, hepatitis and healthy controls, a significant increase was observed (p < 0.001). In conclusion: identification of SNP in COX-2 gene promoter and evaluation of serum AFU and AFP give a red light in early detection of HCC which may be reduce its fatal incidence.

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Authors and Affiliations

  1. Biochemistry Division, Chemistry Department, Faculty of Sciences, Zagazig University, Zagazig, Egypt

    Faten Zahran Mohamed

  2. Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt

    Yousry Mostafa Hussein

  3. Biochemistry Division, Chemistry Department, Faculty of Sciences, Port Said University, Port Said, Egypt

    Ibrahim Mohey El-Deen & Marwa Sayed Sabea

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  1. Faten Zahran Mohamed
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  2. Yousry Mostafa Hussein
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Correspondence to Faten Zahran Mohamed.

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Mohamed, F.Z., Hussein, Y.M., El-Deen, I.M. et al. Cyclooxygenase-2 single-nucleotide polymorphisms and hepatocellular carcinoma in Egypt. Mol Biol Rep 41, 1461–1468 (2014). https://doi.org/10.1007/s11033-013-2991-7

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  • DOI: https://doi.org/10.1007/s11033-013-2991-7

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