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Proteome analysis of roots of wheat seedlings under aluminum stress

Molecular Biology Reports volume 41, pages 671–681 (2014)Cite this article

Abstract

The root apex is considered the first sites of aluminum (Al) toxicity and the reduction in root biomass leads to poor uptake of water and nutrients. Aluminum is considered the most limiting factor for plant productivity in acidic soils. Aluminum is a light metal that makes up 7 % of the earth’s scab dissolving ionic forms. The inhibition of root growth is recognized as the primary effect of Al toxicity. Seeds of wheat cv. Keumkang were germinated on petridish for 5 days and then transferred hydroponic apparatus which was treated without or with 100 and 150 μM AlCl3 for 5 days. The length of roots, shoots and fresh weight of wheat seedlings were decreased under aluminum stress. The concentration of K+, Mg2+ and Ca2+ were decreased, whereas Al3+ and P2O5 − concentration was increased under aluminum stress. Using confocal microscopy, the fluorescence intensity of aluminum increased with morin staining. A proteome analysis was performed to identify proteins, which are responsible to aluminum stress in wheat roots. Proteins were extracted from roots and separated by 2-DE. A total of 47 protein spots were changed under Al stress. Nineteen proteins were significantly increased such as sadenosylmethionine, oxalate oxidase, malate dehydrogenase, cysteine synthase, ascorbate peroxidase and/or, 28 protein spots were significantly decreased such as heat shock protein 70, O-methytransferase 4, enolase, and amylogenin. Our results highlight the importance and identification of stress and defense responsive proteins with morphological and physiological state under Al stress.

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Abbreviations

2-DE:

Two-dimensional electrophoresis

IEF:

Isoelectric focusing

IPG:

Immobilized pH-gradient

LTQ-FTICR:

Linear trap quandropole-fourier transform ion cyclotron resonance

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Acknowledgments

We obtained financial support for this study from the AGENDA (9069532012), RDA, Korea to S. H. Woo, College of Agriculture, Life and Environments, Chungbuk National University, Korea.

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Affiliations

  1. Department of Crop Science, Chungbuk National University, 410 Seongbong-ro, Heungdeok-gu, Cheong-ju, Chungbuk, 361-763, Korea

    Myeong Won Oh, Swapan Kumar Roy & Sun-Hee Woo

  2. National Institute of Crop Science, NARO, Tsukuba, 305-8518, Japan

    Abu Hena Mostofa Kamal & Setsuko Komatsu

  3. Mass Spectrometry Research Center, Korea Basic Science Institute, Chungbuk, Ochang, 863-883, Korea

    Kun Cho

  4. Labaratory of Molecular Breeding, Arid Land Research Center, Tottori University, Tottori, Japan

    Seong-Woo Cho

  5. Department of Crop, Agriculture and Life Science, Chonbuk National University, Jeonju, 561-756, Korea

    Chul-Soo Park

  6. Division of Life Science, Korea Basic Science Institute, Daejon, 305-333, Korea

    Jong-Soon Choi

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  1. Myeong Won Oh
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  2. Swapan Kumar Roy
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  8. Setsuko Komatsu
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  9. Sun-Hee Woo
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Correspondence to Sun-Hee Woo.

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Oh, M.W., Roy, S.K., Kamal, A.H.M. et al. Proteome analysis of roots of wheat seedlings under aluminum stress. Mol Biol Rep 41, 671–681 (2014). https://doi.org/10.1007/s11033-013-2905-8

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  • DOI: https://doi.org/10.1007/s11033-013-2905-8

Keywords

  • Aluminum stress
  • LTQ-FTICR-MS
  • Proteomics
  • Wheat root
  • 2-DE