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Characterization of polymorphic microsatellite markers and genetic diversity in wild bronze featherback, Notopterus notopterus (Pallas, 1769)

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Abstract

Six polymorphic microsatellite DNA loci were identified in the primitive fish, bronze featherback, Notopterus notopterus for the first time and demonstrated significant population genetic structure. Out of the six primers, one primer (NN90) was specific to N. notopterus (microsatellite sequence within the RAG1 gene) and five primers were product of successful cross-species amplification. Sixty-four primers available from 3 fish species of order Osteoglossiformes and families Notopteridae and Osteoglossidae were tested to amplify homologous microsatellite loci in N. notopterus. Fifteen primer pairs exhibited successful cross-priming PCR product. However, polymorphism was detected only at five loci. To assess the significance of these six loci (including NN90) in population genetic study, 215 samples of N. notopterus from five rivers, viz Satluj, Gomti, Yamuna, Brahmaputra and Mahanadi were analyzed. The five sample sets displayed different diversity levels and observed heterozygosity ranged from 0.6036 to 0.7373. Significant genotype heterogeneity (P < 0.0001) and high FST (0.2205) over all loci indicated that the samples are not drawn from the same genepool. The identified microsatellite loci are promising for use in fine-scale population structure analysis of N. notopterus.

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Acknowledgments

The authors express thanks to Director, National Bureau of Fish Genetic Resources (Indian Council of Agricultural Research), Lucknow for supporting the work under institutional project,” Genetic approaches for conservation of prioritized Indian fish species,” Excellent technical assistance provided by Mr. Sree Ram is also duly acknowledged.

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Authors and Affiliations

  1. National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, Lucknow, 226002, Uttar Pradesh, India

    Arti Gupta, Kuldeep K. Lal, Peyush Punia, Rajeev K. Singh, Vindhya Mohindra, Rama S. Sah, Rajesh Kumar, Rupesh K. Luhariya, Arvind K. Dwivedi, Prachi Masih & J. K. Jena

  2. APS University, Rewa, 486001, Madhya Pradesh, India

    R. M. Mishra

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  1. Arti Gupta
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  2. Kuldeep K. Lal
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Correspondence to Kuldeep K. Lal.

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Gupta, A., Lal, K.K., Punia, P. et al. Characterization of polymorphic microsatellite markers and genetic diversity in wild bronze featherback, Notopterus notopterus (Pallas, 1769). Mol Biol Rep 40, 6625–6631 (2013). https://doi.org/10.1007/s11033-013-2776-z

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  • DOI: https://doi.org/10.1007/s11033-013-2776-z

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