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Upregulation of microRNA-146a was not accompanied by downregulation of pro-inflammatory markers in diabetic kidney

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Abstract

The present study was designed to evaluate whether microRNA-146a and its adapter proteins (TRAF6 and IRAK1) are involved in the pathogenesis of diabetes-induced kidney damage. Male Sprague–Dawley rats were divided into control and diabetic groups (n = 6 in each). Diabetes was induced by injection of streptozotocin (55 mg/kg; i.p.) in 12 h fasted rats. Diabetic kidney damage was diagnosed by renal hypertrophy, thickened glomerular basement membrane, widened filtration slits, mesangial expansion, as well as by elevated levels of blood urea and creatinine in diabetic rats 2 months after induction of diabetes. While the expression of NF-κB mRNA and miR-146a were increased in diabetic kidney compared to the sham controls (p < 0.01 for both comparisons), the mRNA levels of IRAK1 and TRAF6 did not statistically reduce. The NF-κB activity and the concentrations of TNF-α, IL-6 and IL-1β in the kidney of diabetic rats were higher than the kidney of controls (p < 0.05 for TNF-α and NF-κB; p < 0.01 for IL-6 and IL-1β). Our results indicate that the upregulation of miR-146a was not accompanied by downregulation of inflammatory mediators in diabetic kidney. It is possible that a defect in the miR-146a-mediated negative loop provides a situation for sustained activation of NF-κB and its targets to promote cells toward abnormalities.

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Acknowledgments

The Grant of this study was supported by Neurosciences Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Our data in this work were derived from the thesis of Ms. Nasibeh Yousefzadeh for a Master of Science degree in physiology (thesis serial number: 90/2-1/1).

Conflict of interest

The authors have declared that there is no conflict of interest.

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Authors and Affiliations

  1. Neurosciences Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Mohammad Reza Alipour & Nasibeh Yousefzadeh

  2. Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

    Mohammad Reza Alipour

  3. Research Center for Pharmaceutical Nanotechnology (RCPN), Tabriz University of Medical Sciences, Tabriz, Iran

    Amir Mahdi Khamaneh & Nasibeh Yousefzadeh

  4. Department of Histology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

    Daryoush Mohammad-nejad

  5. Research Center for Molecular Medicine, Hormozgan University of Medical Sciences, Bandar-Abbas, Iran

    Farhad Ghadiri Soufi

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  1. Mohammad Reza Alipour
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  2. Amir Mahdi Khamaneh
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  3. Nasibeh Yousefzadeh
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  4. Daryoush Mohammad-nejad
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  5. Farhad Ghadiri Soufi
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Correspondence to Farhad Ghadiri Soufi.

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Alipour, M.R., Khamaneh, A.M., Yousefzadeh, N. et al. Upregulation of microRNA-146a was not accompanied by downregulation of pro-inflammatory markers in diabetic kidney. Mol Biol Rep 40, 6477–6483 (2013). https://doi.org/10.1007/s11033-013-2763-4

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  • DOI: https://doi.org/10.1007/s11033-013-2763-4

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