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Molecular phylogeny and biogeography of lac insects (Hemiptera: Kerriidae) inferred from nuclear and mitochondrial gene sequences

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Abstract

Lac insects are commercial scale insects with high economic value. The combined molecular phylogeny of 20 lac insect populations was generated using elongation factor 1 alpha, mitochondrial cytochrome c oxidase subunit I and the small subunit ribosomal RNA gene loci. The 20 populations of lac insects clustered into four distinct clades supported by high bootstrap values in maximum parsimony, maximum likelihood and Bayesian analyses. Clade A at the base of the dendrogram comprises Kerria ruralis and two populations of Kerria lacca and is the branch with most primitive species. Clade B includes K. lacca, Kerria sindica and the three populations P, V and Z from India. They clustered with high bootstrap support and have evolved later than those in Clade A. The three unidentified populations P, V and Z exhibited a close relationship with K. lacca and are the same species. In Clade C, three populations of Kerria yunnanensis (Ym, Yj and Yl), population Ys from Thailand and population H from India clustered as a group, in which population H clustered with Ym with 100 % bootstrap in all three analysis methods. In Clade D, Kerria chinensis, Kerria pusana and three populations of K. yunnanensis clustered together with strong support, and are located in the upper branches of the dendrogram and are recently evolved taxa. The majority of populations from the Indian subcontinent clade are more closely related to outgroup taxa from the primitive family Pseudococcidae, as compared to the Eurasian populations. Phylogenetic analysis reveals that the Indian subcontinent is the centre of original of lac insects which have translocated to the Eurasian Continent. Based on the theory of continental drift and existing fossil records, it is suggested that lac insect evolved from ancient scale insects during the late Cretaceous period when the Indian subcontinent drifted towards the Eurasian Continent. Changes in the global environment have impacted on the distribution and evolution of lac insects during the mid-Cretaceous and early Cenozoic. With increasing temperatures lac insects are likely to translocate to subtropical areas.

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Acknowledgments

The authors want to specially thank Dr. Kevin D. Hyde for reviewing an earlier version of the manuscript. Also we thank Mr. Shaoyun Wang and Shoude Ye for their kind contribution of collecting specimens of lac insects. The voucher specimens (voucher No. 1002-1026) are located in the Museum of Research Institute of Resource Insects, Chinese Academy of Forest, Kunming, Yunnan, China. This work was supported by the National Natural Sciences Foundation of China (No. 30800105, 51175494) and the Grant for Essential Scientific Research of Chinese National Non-profit Institute (No. riricaf201006M) and post-doctor research in Yunnan University.

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Authors and Affiliations

  1. Research Institute of Resource Insect, CAF, Kunming, 650224, China

    Hang Chen, Xiaoming Chen, Ying Feng, Hui Yang, Rui He, Wenfeng Zhang & Zixiang Yang

  2. The Key Laboratory of Cultivating and Utilization of Resources Insects of State Forestry Administration, Kunming, 650224, China

    Xiaoming Chen, Ying Feng & Rui He

  3. Department of Biology, Life Sciences College, Yunnan University, Kunming, 650091, China

    Hang Chen

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  1. Hang Chen
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Correspondence to Xiaoming Chen.

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Chen, H., Chen, X., Feng, Y. et al. Molecular phylogeny and biogeography of lac insects (Hemiptera: Kerriidae) inferred from nuclear and mitochondrial gene sequences. Mol Biol Rep 40, 5943–5952 (2013). https://doi.org/10.1007/s11033-013-2701-5

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