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hABCF3, a TPD52L2 interacting partner, enhances the proliferation of human liver cancer cell lines in vitro

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Abstract

In the present study, we characterized an evolutionarily conserved non-transmembrane ATP-binding cassette protein: hABCF3. Subcellular immunofluorescence staining demonstrated that hABCF3 localizes preferentially in cytoplasm, unlike its paralog protein hABCF1, which localizes in both cytoplasm and nucleus. Quantitative realtime PCR analysis revealed that hABCF3 is expressed in all tissues examined, with high expression level in heart, liver, and pancreas. Interestingly, ectopic hABCF3 promoted proliferation of human liver cancer cell lines. Moreover, knock down of hABCF3 protein expression by siRNA inhibited cell proliferation. In addition, we identified TPD52L2 (Tumor Protein D52-like 2) as a hABCF3 interacting protein via yeast two-hybrid. This interaction was further confirmed by in vivo co-immunoprecipitation and co-localization assays. Furthermore, we identified the interactional region of hABCF3 to be the first 200 amino acids uncharacterized region. Notably, the truncated version of hABCF3, which lacks the TPD52L2 binding region, remarkably impaired hABCF3-mediated cell proliferation. Taken together, these findings suggest that hABCF3 positively regulates cell proliferation, at least partially through the interaction with a tumor protein D52 protein family member: TPD52L2.

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Acknowledgments

This work is supported by grants from the Chinese Natural Science Foundation (30800174) and Science Foundation for Young Scientists of Fudan University to Y. Li, and the National Key Basic Research Program of China (2010CB912603, 2013CB531603), National Special Key Project of China (2008ZX10003-006, 2009ZX09301-011) to K. Huo. We are grateful to Dr. David Shultis and Shirley Lee for proofreading the manuscript and critical discussions. We are also grateful to Dr. Bingbing Wan for providing technical assistance.

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The authors have declared that no conflict of interest exists.

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  1. State Key Laboratory of Genetic Engineering, School of Life Sciences, Institute of Genetics, Fudan University, 220 Handan Rd., Shanghai, 200433, People’s Republic of China

    Juan Zhou, Ying Lin, Huili Shi, Keke Huo & Yanhong Li

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  1. Juan Zhou
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Correspondence to Keke Huo or Yanhong Li.

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Zhou, J., Lin, Y., Shi, H. et al. hABCF3, a TPD52L2 interacting partner, enhances the proliferation of human liver cancer cell lines in vitro. Mol Biol Rep 40, 5759–5767 (2013). https://doi.org/10.1007/s11033-013-2679-z

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