Molecular Biology Reports

, Volume 40, Issue 6, pp 3913–3923 | Cite as

Berberine ameliorates renal injury by regulating G proteins-AC- cAMP signaling in diabetic rats with nephropathy

  • Li Qin Tang
  • Feng Ling Wang
  • Ling Na Zhu
  • Fei Lv
  • Sheng Liu
  • Shan Tang Zhang
Article

Abstract

Diabetic nephropathy (DN) is a progressive kidney disease that is caused by injury to glomerulus and glomerular mesangial cells (MCs) proliferation play a critical role in the pathogenesis of DN. The current studies were undertaken to investigate the protective effects and the possible molecular mechanism of berberine on streptozotocin (STZ)-induced DN rats. Male Wistar rats were randomly assigned to normal control and DN groups of comparable age. Three DN groups received 50, 100 and 200 mg/kg of berberine for 8 weeks via daily intragastrically, respectively. The G proteins-adenylyl cyclase (AC)-cAMP signaling pathway and glomerular MCs proliferation were examined in STZ-induced diabetic rat kidney. Enhanced MCs proliferation and remarkable renal injury were concomitant with activation of Gαi and inhibition of Gαs and cAMP in DN model group. Berberine treatment for 8 weeks abolished the above changes by upregulating the expression of Gαs protein and downregulating the expression of Gαi protein, increasing cAMP level, and inhibiting MCs proliferation compared with model group. Taken together, for the first time, these results demonstrated that berberine can relieve renal injury in DN rats through mediating G proteins-AC-cAMP signaling pathway and inhibiting the abnormal proliferation of MCs by increasing cAMP level, suggesting that berberine could be a potential therapeutic agent for the treatment of DN.

Keywords

Berberine Diabetic nephropathy Cell proliferation G proteins cAMP 

Abbreviations

G proteins

Heterotrimeric GTP-binding proteins

DN

Diabetic nephropathy

STZ

Streptozotocin

ECM

Extracellular matrix

GBM

Glomerular basement membrane

Camp

Adenosine 3-,5- monophosphate

AC

Adenylate cyclase

TGF-β1

Transforming growth factor-beta1

FN

Fibronectin

CTGF

Connective tissue factor

FBG

Fasting blood glucose

Notes

Acknowledgments

This Project was supported by the National Natural Science Foundation of China (Nos. 81073109, 81102864) and Natural Science Foundation of Anhui Province (China, No. 090413106). We would like to extend our appreciation to Wei Wei and the staff at the School of Pharmacy, Anhui College of Traditional Chinese Medicine for their technical support.

Conflict of interest

There are no competing financial interests in relation to the work.

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Li Qin Tang
    • 1
  • Feng Ling Wang
    • 2
  • Ling Na Zhu
    • 1
  • Fei Lv
    • 1
  • Sheng Liu
    • 1
  • Shan Tang Zhang
    • 1
  1. 1.Department of PharmacyAffiliated Anhui Provincial Hospital, Anhui Medical UniversityHefeiPeople’s Republic of China
  2. 2.Institute of Clinical Pharmacology, Anhui Medical UniversityHefeiPeople’s Republic of China

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