We investigated the genetic markers of twelve X-STR loci in 670 healthy, unrelated Japanese (438 men and 232 women) from Tokyo and 488 Chinese (263 men and 225 women) from Shenyang, using the Investigator Argus X-12 kit. Allele and haplotype analyses of twelve X-STRs clustered into four linkage groups indicated that they are highly informative for forensic applications in Japanese and Chinese populations. Hardy–Weinberg equilibrium tests demonstrated no significant deviations in the two populations. Among the four closely linked X-STR trios, some haplotype unique to Japanese or Chinese population were detected. Haplotype diversity for each linkage group ranged from 0.9861 to 0.9968, showing high values in each of the study populations. The genetic distances between populations based on the 12 X-STR loci and the phylogenetic tree revealed long genetic distances between Asian and Caucasian populations and between Asian and African population (Moroccan). These results suggest that the twelve X-STR loci will contribute to forensic casework in Japanese and Chinese populations.
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Szibor R, Krawczak M, Hering S, Edelmann J, Kuhlisch E, Krause D (2003) Use of X-linked markers for forensic purposes. Int J Legal Med 117:67–74
Freitas NS, Resque RL, Ribeiro-Rodrigues EM, Guerreiro JF, Santos NP, Ribeiro-dos-Santos A (2010) X-linked insertion/deletion polymorphisms: forensic applications of a 33-markers panel. Int J Legal Med 124:589–593
Samejima M, Nakamura Y, Minaguchi K (2011) Population genetic study of six closely linked groups of X-STRs in a Japanese population. Int J Legal Med 125:895–900
Li C, Ma T, Zhao S, Zhang S, Xu J, Zhao Z (2011) Development of 11 X-STR loci typing system and genetic analysis in Tibetan and Northern Han populations from China. Int J Legal Med 125:753–756
Tie J, Uchigasaki S, Oshida S (2010) Genetic polymorphisms of eight X-chromosomal STR loci in the population of Japanese. Forensic Sci Int Genet 4(4):e105–e108
QIAGEN Sample and Assay Technologies (2010): Investigator Argus X-12 Handbook
Czarnogórska M, Sanak M, Piniewska D, Polańska N, Stawowiak A, Opolska-Bogusz B (2010) Identification of rare genetic variants at DXS10074, DXS10079, DXS10146 and DXS10148 loci of investigator Argus X-12 multiplex in the south polish population. Arch Med Sadowej Kryminol 60:235–242
Ribeiro-Rodrigues EM, Palha Tde J, Bittencourt EA, Ribeiro-Dos-Santos A, Santos S (2011) Extensive survey of 12 X-STRs reveals genetic heterogeneity among Brazilian populations. Int J Legal Med 125:445–452
Takezaki N, Nei M, Tamura K (2010) POPTREE2: software for constructing population trees from allele frequency data and computing other population statistics with windows interface. Mol Biol Evol 27(4):747–752
Excoffier L, Laval G, Schneider S (2005) Arlequin ver. 3.0: an integrated software package for population genetics data analysis. Evol Bioinform Online 1:47–50
Sim JE, Lee HY, Yang WI, Shin KJ (2010) Population genetic study of four closely-linked X-STR trios in Koreans. Mol Biol Rep 37:333–337
Zhang S, Zhao S, Zhu R, Li CT (2012) Genetic polymorphisms of 12 X-STR for forensic purposes in Shanghai Han population from China. Mol Biol Rep 39:5705–5707
Edelmann J, Lutz-Bonengel S, Naue J, Hering S (2012) X-chromosomal haplotype frequencies of four linkage groups using the investigator Argus X-12 Kit. Forensic Sci Int Genet 6:e24–e34
Horváth G, Zalán A, Kis Z, Pamjav H (2012) A genetic study of 12 X-STR loci in the Hungarian population. Forensic Sci Int Genet 6:e46–e47
Tillmar AO (2012) Population genetic analysis of 12 X-STRs in Swedish population. Forensic Sci Int Genet 6:e80–e81
Bentayebi K, Picornell A, Bouabdeallah M, Castro JA, Aboukhalid R, Squalli D (2012) Genetic diversity of 12 X-chromosomal short tandem repeats in the Moroccan population. Forensic Sci Int Genet 6:e48–e49
Tomas C, Pereira V, Morling N (2012) Analysis of 12 X-STRs in Greenlanders, Danes and Somalis using Argus X-12. Int J Legal Med 126(1):121–128
Hundertmark T, Hering S, Edelmann J, Augustin C, Plate I, Szibor R (2008) The STR cluster DXS10148-DXS8378-DXS10135 provides a powerful tool for X-chromosomal haplotyping at Xp22. Int J Legal Med 122:489–492
We thank Tamiko Nishino, Kimiko Semiya, Noriko Son and Hiroko Abe who assisted with the experiments of this study.
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Uchigasaki, S., Tie, J. & Takahashi, D. Genetic analysis of twelve X-chromosomal STRs in Japanese and Chinese populations. Mol Biol Rep 40, 3193–3196 (2013). https://doi.org/10.1007/s11033-012-2394-1
- X chromosome
- Genetic diversity