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Molecular Biology Reports

, Volume 40, Issue 2, pp 1429–1441 | Cite as

Overexpressed C-type natriuretic peptide serves as an early compensatory response to counteract extracellular matrix remodeling in unilateral ureteral obstruction rats

  • Peng HuEmail author
  • Jing Wang
  • Xue Qi Zhao
  • Bo Hu
  • Ling Lu
  • Yuan Han Qin
Article

Abstract

Although the mechanism underlying C-type natriuretic peptide (CNP) beneficial effects is not entirely understood, modulating the expression of matrix metalloproteinases (MMPs)/tissue inhibitors of metalloproteinases (TIMPs) may play an important role. The study presented herein was designed as a first demonstration of the regulative effects of CNP on MMPs/TIMPs expression in unilateral ureteral obstruction (UUO) rats. The continuous changes of CNP, MMP-2, MMP-9, TIMP-1, TIMP-2 and type IV collagen (Col-IV) expression were determined in the obstructed rat kidneys at 3 days, 1, 2, and 3 months post-UUO respectively. According to the real-time PCR analysis, CNP, MMP-2 and MMP-9 mRNA expression in the obstructed kidneys were significantly higher compared to every time corresponding SOR, and progressively decreased over time. In contrast, in the obstructed kidneys collected 2 and 3 months post-UUO, the higher TIMP-1 and TIMP-2 mRNA expression were observed in comparison to the corresponding SOR group. The above trends of CNP, MMP-2, MMP-9, TIMP-1, and TIMP-2 transcripts were confirmed by their protein expression based on immunohistochemistry and western blot, and finally contributed to the progressive elevated Col-IV expression in the obstructed kidneys throughout the entire study period. Overexpressed CNP may be an early compensatory response to counteract extracellular matrix remodeling in UUO rats.

Keywords

C-type natriuretic peptide Matrix metalloproteinases Epithelial-mesenchymal transdifferentiation Extracellular matrix Tubulointerstitial fibrosis Wistar rat 

Notes

Acknowledgments

This study was supported by the National Natural Science Foundation of China (No. 81000306) and the Post-Doctoral Foundation of Anhui Medical University (No. 2009KJ02).

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Peng Hu
    • 1
    Email author
  • Jing Wang
    • 1
  • Xue Qi Zhao
    • 1
  • Bo Hu
    • 1
  • Ling Lu
    • 1
  • Yuan Han Qin
    • 2
  1. 1.Department of PediatricsThe First Affiliated Hospital of Anhui Medical UniversityHefeiPeople’s Republic of China
  2. 2.Department of PediatricsThe First Affiliated Hospital of Guangxi Medical UniversityNanningPeople’s Republic of China

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